The aim of this work was to test different molecular techniques for determining which was the most effective in the identification of Saccharomyces cerevisiae strains. In particular, polymerase chain reaction--restriction fragment length polymorphism (PCR-RFLP) analysis of the internal transcribed spacer (ITS) regions and the nontranscribed spacer 2 (NTS2) region, sequencing of the D1/D2 domain, and electrophoretic karyotyping were applied to 123 yeast strains isolated from different sourdoughs and tentatively attributed to the species S. cerevisiae. All of the strains tested showed an identical PCR-RFLP pattern for the ITS regions, an identical nucleotide sequence of the D1/D2 domain, and the typical electrophoretic karyo type of S. cerevisiae. In contrast, 14 out of the 123 strains tested showed some polymorphism with BanI restriction analysis of the NTS2 region. Our results indicate that while the sequencing of the D1/D2 domain, the PCR-RFLP analysis of the ITS regions, and the electrophoretic karyotype can be employed successfully to identify S. cerevisiae strains, PCR-RFLP analysis of the NTS2 region does not allow a consistent and accurate grouping for S. cerevisiae strains. The fact that the NTS2 region of a small number of strains (8.78% of the total strains tested) is different from that of the other S. cerevisiae strains confirms that molecular methods should always be tested on a great number of strains.

Limits of r-DNA-NTS2 regions on the taxonomy of Saccharomyces genus / Pulvirenti, Andrea; Solieri, Lisa; DE VERO, Luciana; Gullo, Maria; Giudici, Paolo. - STAMPA. - (2004), pp. 131-131. ((Intervento presentato al convegno 11th International Congress on Yeasts. Yeasts in Sciences and Biotechnology tenutosi a Rio de Janeiro - Brasil nel August 15th to 20th, 2004.

Limits of r-DNA-NTS2 regions on the taxonomy of Saccharomyces genus

PULVIRENTI, Andrea;SOLIERI, lisa;DE VERO, Luciana;GULLO, Maria;GIUDICI, Paolo
2004

Abstract

The aim of this work was to test different molecular techniques for determining which was the most effective in the identification of Saccharomyces cerevisiae strains. In particular, polymerase chain reaction--restriction fragment length polymorphism (PCR-RFLP) analysis of the internal transcribed spacer (ITS) regions and the nontranscribed spacer 2 (NTS2) region, sequencing of the D1/D2 domain, and electrophoretic karyotyping were applied to 123 yeast strains isolated from different sourdoughs and tentatively attributed to the species S. cerevisiae. All of the strains tested showed an identical PCR-RFLP pattern for the ITS regions, an identical nucleotide sequence of the D1/D2 domain, and the typical electrophoretic karyo type of S. cerevisiae. In contrast, 14 out of the 123 strains tested showed some polymorphism with BanI restriction analysis of the NTS2 region. Our results indicate that while the sequencing of the D1/D2 domain, the PCR-RFLP analysis of the ITS regions, and the electrophoretic karyotype can be employed successfully to identify S. cerevisiae strains, PCR-RFLP analysis of the NTS2 region does not allow a consistent and accurate grouping for S. cerevisiae strains. The fact that the NTS2 region of a small number of strains (8.78% of the total strains tested) is different from that of the other S. cerevisiae strains confirms that molecular methods should always be tested on a great number of strains.
11th International Congress on Yeasts. Yeasts in Sciences and Biotechnology
Rio de Janeiro - Brasil
August 15th to 20th, 2004
131
131
Pulvirenti, Andrea; Solieri, Lisa; DE VERO, Luciana; Gullo, Maria; Giudici, Paolo
Limits of r-DNA-NTS2 regions on the taxonomy of Saccharomyces genus / Pulvirenti, Andrea; Solieri, Lisa; DE VERO, Luciana; Gullo, Maria; Giudici, Paolo. - STAMPA. - (2004), pp. 131-131. ((Intervento presentato al convegno 11th International Congress on Yeasts. Yeasts in Sciences and Biotechnology tenutosi a Rio de Janeiro - Brasil nel August 15th to 20th, 2004.
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11380/308077
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