Genus Zygosaccharomyces includes osmo/halotolerant yeasts that diverged from the S. cerevisiae lineage prior to its genome duplication (WGD). Recently, we delineated a novel species, Zygosaccharomyces sapae. Z. sapae has phylogenetic importance as its conspecific strain NCYC3042 has been implicated in allodiploid nature of hybrid ATCC42981. We carry out an extensive genetic survey on some Zygosaccharomyces strains (called Z. rouxii complex) to unravel intra and inter-strain DNA sequences heterogeneity; and diversity in chromosome patterns. Intragenomic copies of rDNA genes are not homogenized by concerted evolution in them, with indels and substitutions occurring mainly in internal transcribed spacer regions. This suggests a birth and death process of evolution. Variability in protein-coding sequences and ploidy level showed a marked intergenomic diversity in diploid and aneuploid cells. Functional analysis revealed a broad range tolerance to alkali metal cations, congruent with clusters obtained through genetic survey. Life cycle and breeding system are suggested to shape these diversities in this complex, making it an interesting pre-WGD model for studying evolutionary forces driving genome plasticity in low-aw environments.
Getting insights from genomic complexities in Zygosaccharomyces rouxii complex / Dakal, TIKAM CHAND; Solieri, Lisa; Giudici, Paolo. - In: YEAST. - ISSN 0749-503X. - 30:(2013), pp. 232-232. (Intervento presentato al convegno 26th International Conference on Yeast Genetics and Molecular Biology tenutosi a Frankfurt, Germany, nel 29th August-3rd September 2013) [10.1002/yea.2974].
Getting insights from genomic complexities in Zygosaccharomyces rouxii complex
DAKAL, TIKAM CHAND;SOLIERI, lisa;GIUDICI, Paolo
2013
Abstract
Genus Zygosaccharomyces includes osmo/halotolerant yeasts that diverged from the S. cerevisiae lineage prior to its genome duplication (WGD). Recently, we delineated a novel species, Zygosaccharomyces sapae. Z. sapae has phylogenetic importance as its conspecific strain NCYC3042 has been implicated in allodiploid nature of hybrid ATCC42981. We carry out an extensive genetic survey on some Zygosaccharomyces strains (called Z. rouxii complex) to unravel intra and inter-strain DNA sequences heterogeneity; and diversity in chromosome patterns. Intragenomic copies of rDNA genes are not homogenized by concerted evolution in them, with indels and substitutions occurring mainly in internal transcribed spacer regions. This suggests a birth and death process of evolution. Variability in protein-coding sequences and ploidy level showed a marked intergenomic diversity in diploid and aneuploid cells. Functional analysis revealed a broad range tolerance to alkali metal cations, congruent with clusters obtained through genetic survey. Life cycle and breeding system are suggested to shape these diversities in this complex, making it an interesting pre-WGD model for studying evolutionary forces driving genome plasticity in low-aw environments.Pubblicazioni consigliate
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