Rhodotorula glacialis AS 4.7 is an oleaginous psychrophilic yeast which was isolated from glacial environments. Despite its origin, the strain abundantly grew and accumulated lipids up to 20°C. The growth temperature did not influence the yield coefficients of both biomass and lipids production, but had significant effects on the growth rate and thus on volumetric productivity of lipid. 15°C were identified as the optimum temperature for lipid production. As the growth temperature decreased, the abundance of C18 fatty acids (FA) increased at the expenses of C16 FA and the unsaturation degree increased as well. In particular, remarkable amounts of linolenic acid (C18:3 ω-3) were produced at -3°C, accounting for 29% of FA. Lipid production by R. glacialis AS 4.7 especially occurred in carbon rich media, through a two-stages process. The first stage resulted in multiplication of cells and finished with the exhaustion of a nutrient other than the carbon source. During the second stage, the excess glucose was converted into intracellular storage lipids. The extent of the carbon excess had major positive effects on lipid production. The lipid content of biomass, glucose conversion into lipids, lipid concentration, and lipids productivity were all maximum with 120 g L-1 glucose (68%, 16%, 19 g L-1, and 0.054 g L-1 h-1, respectively). The results herein reported suggest that R. glacialis AS 4.7 could be considered as an interesting microorganism for the production of single cell oils and represent the first proposed biotechnological application for this yeast species.

Production of Single Cell Oils by the Cold-Adapted Oleaginous Yeast Rhodotorula glacialis AS 4.7: Effects of the Growth Temperature and the C:N Ratio / Amaretti, Alberto; Raimondi, Stefano; Sala, Maurizio; Roncaglia, Lucia; DE LUCIA, Marzia; Leonardi, Alan; Rossi, Maddalena. - STAMPA. - 20:(2010), pp. 109-114. ((Intervento presentato al convegno IBIC2010: 2ND INTERNATIONAL CONFERENCE ON INDUSTRIAL BIOTECHNOLOGY tenutosi a Padova nel 2010 [10.3303/CET1020019].

Production of Single Cell Oils by the Cold-Adapted Oleaginous Yeast Rhodotorula glacialis AS 4.7: Effects of the Growth Temperature and the C:N Ratio.

AMARETTI, Alberto;RAIMONDI, Stefano;SALA, Maurizio;RONCAGLIA, Lucia;DE LUCIA, MARZIA;LEONARDI, Alan;ROSSI, Maddalena
2010

Abstract

Rhodotorula glacialis AS 4.7 is an oleaginous psychrophilic yeast which was isolated from glacial environments. Despite its origin, the strain abundantly grew and accumulated lipids up to 20°C. The growth temperature did not influence the yield coefficients of both biomass and lipids production, but had significant effects on the growth rate and thus on volumetric productivity of lipid. 15°C were identified as the optimum temperature for lipid production. As the growth temperature decreased, the abundance of C18 fatty acids (FA) increased at the expenses of C16 FA and the unsaturation degree increased as well. In particular, remarkable amounts of linolenic acid (C18:3 ω-3) were produced at -3°C, accounting for 29% of FA. Lipid production by R. glacialis AS 4.7 especially occurred in carbon rich media, through a two-stages process. The first stage resulted in multiplication of cells and finished with the exhaustion of a nutrient other than the carbon source. During the second stage, the excess glucose was converted into intracellular storage lipids. The extent of the carbon excess had major positive effects on lipid production. The lipid content of biomass, glucose conversion into lipids, lipid concentration, and lipids productivity were all maximum with 120 g L-1 glucose (68%, 16%, 19 g L-1, and 0.054 g L-1 h-1, respectively). The results herein reported suggest that R. glacialis AS 4.7 could be considered as an interesting microorganism for the production of single cell oils and represent the first proposed biotechnological application for this yeast species.
IBIC2010: 2ND INTERNATIONAL CONFERENCE ON INDUSTRIAL BIOTECHNOLOGY
Padova
2010
20
109
114
Amaretti, Alberto; Raimondi, Stefano; Sala, Maurizio; Roncaglia, Lucia; DE LUCIA, Marzia; Leonardi, Alan; Rossi, Maddalena
Production of Single Cell Oils by the Cold-Adapted Oleaginous Yeast Rhodotorula glacialis AS 4.7: Effects of the Growth Temperature and the C:N Ratio / Amaretti, Alberto; Raimondi, Stefano; Sala, Maurizio; Roncaglia, Lucia; DE LUCIA, Marzia; Leonardi, Alan; Rossi, Maddalena. - STAMPA. - 20:(2010), pp. 109-114. ((Intervento presentato al convegno IBIC2010: 2ND INTERNATIONAL CONFERENCE ON INDUSTRIAL BIOTECHNOLOGY tenutosi a Padova nel 2010 [10.3303/CET1020019].
File in questo prodotto:
File Dimensione Formato  
2010 Padova.pdf

accesso aperto

Descrizione: full text
Tipologia: Versione dell'editore (versione pubblicata)
Dimensione 655.82 kB
Formato Adobe PDF
655.82 kB Adobe PDF Visualizza/Apri
Pubblicazioni consigliate

Caricamento pubblicazioni consigliate

Licenza Creative Commons
I metadati presenti in IRIS UNIMORE sono rilasciati con licenza Creative Commons CC0 1.0 Universal, mentre i file delle pubblicazioni sono rilasciati con licenza Attribuzione 4.0 Internazionale (CC BY 4.0), salvo diversa indicazione.
In caso di violazione di copyright, contattare Supporto Iris

Utilizza questo identificativo per citare o creare un link a questo documento: http://hdl.handle.net/11380/861736
Citazioni
  • ???jsp.display-item.citation.pmc??? ND
  • Scopus 6
  • ???jsp.display-item.citation.isi??? 3
social impact