The crossing of Saccharomyces strains by spore conjugation is one of the ways toobtain new starter cultures for the fermentation industry. One of the majordifficulties of this practice is the identification of the newly formed hybrids. In thiswork we describe an effective molecular method for the validation ofSaccharomyces intraspecific crosses. The method described is based in thehypothesis that hybrids constructed by spore conjugation contain the sum of thegenomes of both parental strains. As a consequence, the conjugation of spores oftwo yeasts showing different genomic fingerprinting profiles will result in a hybridculture that will show the sum of both profiles. In this work wWe demonstrated thatthe detection of polymorphism in two genes containing minisatellite-like sequences;,either SED1 or AGA1, is suitable for this purpose. Using this strategy we were ableto validate 15 crosses out of 162 hybridization attempts.
Method for the validation of intraspecific crosses of Saccharomycescerevisiae strains by minisatellite analysis / Boveri, Silvio; Rainieri, Sandra; Pulvirenti, Andrea. - In: CANADIAN JOURNAL OF MICROBIOLOGY. - ISSN 0008-4166. - STAMPA. - 58:3(2012), pp. 350-358. [10.1139/W11-142]
Method for the validation of intraspecific crosses of Saccharomycescerevisiae strains by minisatellite analysis
BOVERI, SILVIO;RAINIERI, SANDRA;PULVIRENTI, Andrea
2012
Abstract
The crossing of Saccharomyces strains by spore conjugation is one of the ways toobtain new starter cultures for the fermentation industry. One of the majordifficulties of this practice is the identification of the newly formed hybrids. In thiswork we describe an effective molecular method for the validation ofSaccharomyces intraspecific crosses. The method described is based in thehypothesis that hybrids constructed by spore conjugation contain the sum of thegenomes of both parental strains. As a consequence, the conjugation of spores oftwo yeasts showing different genomic fingerprinting profiles will result in a hybridculture that will show the sum of both profiles. In this work wWe demonstrated thatthe detection of polymorphism in two genes containing minisatellite-like sequences;,either SED1 or AGA1, is suitable for this purpose. Using this strategy we were ableto validate 15 crosses out of 162 hybridization attempts.
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