A liquid chromatography-tandem mass spectrometry method for the determination of four Alternaria toxins, i.e. alternariol, alternariol monomethyl ether, tentoxin, and tenuazonic acid in tomato-based and fruit-based products was developed using a QuEChERs approach for the extraction of the mycotoxins. To optimise the QuEChERs extraction, several parameters were tested: types of QuEChERs pouches, sample weights, quantities of added water, use of dispersive SPE as a purification step, types of solvent and conditions of shaking. The method showed good linearity (R2> 0.997) and precision (RSD% < 10) for all analytes. Tenuazonic acid showed very good recovery (98.8%-108.9%) for tomato-based products, as well as for fruit-based products. The method was successfully applied to 57 samples collected from the Italian market. Tenuazonic acid was found in appreciable concentrations in some products. The highest value was found in a tomato sauce sample (814 μg/kg).

Determination of four Alternaria alternata mycotoxins by QuEChERS approach coupled with liquid chromatography-tandem mass spectrometry in tomato-based and fruit-based products / De Berardis, Sara; De Paola, Eleonora Laura; Montevecchi, Giuseppe; Garbini, Davide; Masino, Francesca; Antonelli, Andrea; Melucci, Dora. - In: FOOD RESEARCH INTERNATIONAL. - ISSN 0963-9969. - 106:(2018), pp. 677-685. [10.1016/j.foodres.2018.01.032]

Determination of four Alternaria alternata mycotoxins by QuEChERS approach coupled with liquid chromatography-tandem mass spectrometry in tomato-based and fruit-based products

De Paola, Eleonora Laura;Montevecchi, Giuseppe
;
Masino, Francesca;Antonelli, Andrea;
2018

Abstract

A liquid chromatography-tandem mass spectrometry method for the determination of four Alternaria toxins, i.e. alternariol, alternariol monomethyl ether, tentoxin, and tenuazonic acid in tomato-based and fruit-based products was developed using a QuEChERs approach for the extraction of the mycotoxins. To optimise the QuEChERs extraction, several parameters were tested: types of QuEChERs pouches, sample weights, quantities of added water, use of dispersive SPE as a purification step, types of solvent and conditions of shaking. The method showed good linearity (R2> 0.997) and precision (RSD% < 10) for all analytes. Tenuazonic acid showed very good recovery (98.8%-108.9%) for tomato-based products, as well as for fruit-based products. The method was successfully applied to 57 samples collected from the Italian market. Tenuazonic acid was found in appreciable concentrations in some products. The highest value was found in a tomato sauce sample (814 μg/kg).
8-feb-2018
106
677
685
Determination of four Alternaria alternata mycotoxins by QuEChERS approach coupled with liquid chromatography-tandem mass spectrometry in tomato-based and fruit-based products / De Berardis, Sara; De Paola, Eleonora Laura; Montevecchi, Giuseppe; Garbini, Davide; Masino, Francesca; Antonelli, Andrea; Melucci, Dora. - In: FOOD RESEARCH INTERNATIONAL. - ISSN 0963-9969. - 106:(2018), pp. 677-685. [10.1016/j.foodres.2018.01.032]
De Berardis, Sara; De Paola, Eleonora Laura; Montevecchi, Giuseppe; Garbini, Davide; Masino, Francesca; Antonelli, Andrea; Melucci, Dora
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Utilizza questo identificativo per citare o creare un link a questo documento: http://hdl.handle.net/11380/1155139
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