Scope: Chlorogenic acid (3-O-caffeoyl-quinic acid, C-QA), the caffeic ester of quinic acid, is one of the most abundant phenolic acids in Western diet. The majority of C-QA escapes absorption in the small intestine and reaches the colon, where the resident microbiota transforms it into several metabolites. C-QA conversion by the gut microbiota from nine subjects was compared to evaluate the variability of bacterial metabolism. It was investigated whether a potentially probiotic Bifidobacterium strain, capable of C-QA hydrolysis, could affect C-QA fate. Methods and results: Bioconversion experiments exploiting the microbiota from diverse subjects revealed that C-QA was metabolized through a succession of hydrogenation, dexydroxylation and ester hydrolysis, occurring in different order among the subjects. Transformation may proceed also through quinic acid residue breakdown, since caffeoyl-glycerol intermediates were identified (HPLC-MS/MS, Q-TOF). All the pathways converged on 3-(3-hydroxyphenyl)-propanoic acid, which was transformed to hydroxyphenyl-ethanol and/or phenylacetic acid in few subjects. A strain of Bifidobacterium animalis able to hydrolyze C-QA was added to microbiota cultures. It affected microbial composition but not to such an extent that C-QA metabolism was modified. Conclusion: A picture of the variability of microbiota C-QA transformations among subjects is provided. The transformation route through caffeoyl-glycerol intermediates is described for the first time.

In vitro transformation of chlorogenic acid by human gut microbiota / Francisco, Tomas Barberan; Rocío, García Villalba; Quartieri, Andrea; Raimondi, Stefano; Amaretti, Alberto; Leonardi, Alan; Rossi, Maddalena. - In: MOLECULAR NUTRITION & FOOD RESEARCH. - ISSN 1613-4125. - STAMPA. - 58:(2014), pp. 1122-1131. [10.1002/mnfr.201300441]

In vitro transformation of chlorogenic acid by human gut microbiota

QUARTIERI, ANDREA;RAIMONDI, Stefano;AMARETTI, Alberto;LEONARDI, Alan;ROSSI, Maddalena
2014

Abstract

Scope: Chlorogenic acid (3-O-caffeoyl-quinic acid, C-QA), the caffeic ester of quinic acid, is one of the most abundant phenolic acids in Western diet. The majority of C-QA escapes absorption in the small intestine and reaches the colon, where the resident microbiota transforms it into several metabolites. C-QA conversion by the gut microbiota from nine subjects was compared to evaluate the variability of bacterial metabolism. It was investigated whether a potentially probiotic Bifidobacterium strain, capable of C-QA hydrolysis, could affect C-QA fate. Methods and results: Bioconversion experiments exploiting the microbiota from diverse subjects revealed that C-QA was metabolized through a succession of hydrogenation, dexydroxylation and ester hydrolysis, occurring in different order among the subjects. Transformation may proceed also through quinic acid residue breakdown, since caffeoyl-glycerol intermediates were identified (HPLC-MS/MS, Q-TOF). All the pathways converged on 3-(3-hydroxyphenyl)-propanoic acid, which was transformed to hydroxyphenyl-ethanol and/or phenylacetic acid in few subjects. A strain of Bifidobacterium animalis able to hydrolyze C-QA was added to microbiota cultures. It affected microbial composition but not to such an extent that C-QA metabolism was modified. Conclusion: A picture of the variability of microbiota C-QA transformations among subjects is provided. The transformation route through caffeoyl-glycerol intermediates is described for the first time.
58
1122
1131
In vitro transformation of chlorogenic acid by human gut microbiota / Francisco, Tomas Barberan; Rocío, García Villalba; Quartieri, Andrea; Raimondi, Stefano; Amaretti, Alberto; Leonardi, Alan; Rossi, Maddalena. - In: MOLECULAR NUTRITION & FOOD RESEARCH. - ISSN 1613-4125. - STAMPA. - 58:(2014), pp. 1122-1131. [10.1002/mnfr.201300441]
Francisco, Tomas Barberan; Rocío, García Villalba; Quartieri, Andrea; Raimondi, Stefano; Amaretti, Alberto; Leonardi, Alan; Rossi, Maddalena
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Utilizza questo identificativo per citare o creare un link a questo documento: http://hdl.handle.net/11380/1023717
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