Aim: In this study, we attempted to identify the proteins involved in periodontitis comparing the proteomic profile of interproximal pocket tissue affected by the periodontal lesion with interproximal healthy tissue in the same subject, in sites where no periodontopathogenic bacteria were detectable. Material and Methods: Using specific inclusion and exclusion criteria, we enrolled 15 subjects affected by severe chronic periodontitis. The subjects presenting at least one intrabony defect (next to a healthy interproximal site to be included in the flap design) suitable for treatment by osseous respective surgery, were considered eligible for this study. Biopsies of connective tissue were harvested from the intrabony component of the defect and from healthy tissue (from the secondary flap) and immediately frozen at –80°C. A two-dimensional gel electrophoresis system was used to detect differences in protein expression between pathologic and healthy tissue and protein identification by LC-MS/MS analysis was performed. Results: Six proteins (tropomyosin α-4 chain, 14-3-3 protein z/δ, putative heat shock protein HSP 90-β, peroxiredoxin-1, fatty acid binding protein, S100-A9) displayed a higher while five a lower (annexin A1, actin cytoplasmatic-1, carbonic anhydrase-2, Ig K chain C region and flavin reductase) protein expression level in pathologic tissue compared with healthy tissue. Conclusion: The highlight of proteins involved in the immunological and cytokine signaling cascade mediation or in connective and epithelial regeneration and differentiation shows that the pathogenic process is active in periodontal sites also in absence of periodontopathogen microbiota. Besides, proteomic analysis could be considered very useful in studying the pathogenesis of periodontitis. Abstract N: P0224 ISSN:1600-051X (Electronic).

Proteomic profile of non-bacterial proteins in periodontal pockets: a pilot study / Simonazzi, L.; Monari, Emanuela; Ferrari, Davide; Bellei, Elisa; Melpignano, F.; Cuoghi, Aurora; Bergamini, Stefania; Pellacani, C.; Campedelli, F.; Tomasi, Aldo; Bertoldi, Carlo. - In: JOURNAL OF CLINICAL PERIODONTOLOGY. - ISSN 0303-6979. - STAMPA. - 39 (Suppl. 13):(2012), pp. 144-144. ((Intervento presentato al convegno EUROPERIO 7 tenutosi a Vienna - Austria nel June 6-9, 2012.

Proteomic profile of non-bacterial proteins in periodontal pockets: a pilot study.

MONARI, Emanuela;FERRARI, Davide;BELLEI, Elisa;CUOGHI, Aurora;BERGAMINI, Stefania;TOMASI, Aldo;BERTOLDI, Carlo
2012-01-01

Abstract

Aim: In this study, we attempted to identify the proteins involved in periodontitis comparing the proteomic profile of interproximal pocket tissue affected by the periodontal lesion with interproximal healthy tissue in the same subject, in sites where no periodontopathogenic bacteria were detectable. Material and Methods: Using specific inclusion and exclusion criteria, we enrolled 15 subjects affected by severe chronic periodontitis. The subjects presenting at least one intrabony defect (next to a healthy interproximal site to be included in the flap design) suitable for treatment by osseous respective surgery, were considered eligible for this study. Biopsies of connective tissue were harvested from the intrabony component of the defect and from healthy tissue (from the secondary flap) and immediately frozen at –80°C. A two-dimensional gel electrophoresis system was used to detect differences in protein expression between pathologic and healthy tissue and protein identification by LC-MS/MS analysis was performed. Results: Six proteins (tropomyosin α-4 chain, 14-3-3 protein z/δ, putative heat shock protein HSP 90-β, peroxiredoxin-1, fatty acid binding protein, S100-A9) displayed a higher while five a lower (annexin A1, actin cytoplasmatic-1, carbonic anhydrase-2, Ig K chain C region and flavin reductase) protein expression level in pathologic tissue compared with healthy tissue. Conclusion: The highlight of proteins involved in the immunological and cytokine signaling cascade mediation or in connective and epithelial regeneration and differentiation shows that the pathogenic process is active in periodontal sites also in absence of periodontopathogen microbiota. Besides, proteomic analysis could be considered very useful in studying the pathogenesis of periodontitis. Abstract N: P0224 ISSN:1600-051X (Electronic).
39 (Suppl. 13)
144
144
Simonazzi, L.; Monari, Emanuela; Ferrari, Davide; Bellei, Elisa; Melpignano, F.; Cuoghi, Aurora; Bergamini, Stefania; Pellacani, C.; Campedelli, F.; Tomasi, Aldo; Bertoldi, Carlo
Proteomic profile of non-bacterial proteins in periodontal pockets: a pilot study / Simonazzi, L.; Monari, Emanuela; Ferrari, Davide; Bellei, Elisa; Melpignano, F.; Cuoghi, Aurora; Bergamini, Stefania; Pellacani, C.; Campedelli, F.; Tomasi, Aldo; Bertoldi, Carlo. - In: JOURNAL OF CLINICAL PERIODONTOLOGY. - ISSN 0303-6979. - STAMPA. - 39 (Suppl. 13):(2012), pp. 144-144. ((Intervento presentato al convegno EUROPERIO 7 tenutosi a Vienna - Austria nel June 6-9, 2012.
File in questo prodotto:
File Dimensione Formato  
2012 Bertoldi EUROPERIO 7 JCP.pdf

Accesso riservato

Tipologia: Versione dell'autore revisionata e accettata per la pubblicazione
Dimensione 3.3 MB
Formato Adobe PDF
3.3 MB Adobe PDF   Visualizza/Apri   Richiedi una copia
Pubblicazioni consigliate

Licenza Creative Commons
I metadati presenti in IRIS UNIMORE sono rilasciati con licenza Creative Commons CC0 1.0 Universal, mentre i file delle pubblicazioni sono rilasciati con licenza Attribuzione 4.0 Internazionale (CC BY 4.0), salvo diversa indicazione.
In caso di violazione di copyright, contattare Supporto Iris

Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11380/807496
Citazioni
  • ???jsp.display-item.citation.pmc??? ND
  • Scopus ND
  • ???jsp.display-item.citation.isi??? ND
social impact