To investigate how much insulin-like growth factor I may modulate the LH response to GnRH.In primary cultures of male rat anterior pituitary cells, the LH response to GnRH (10(-9) M) was evaluated after a 24-hour preincubation with medium alone, IGF-I (1, 5, 10, 15 nM), polyclonal antibody anti-IGF-I (Ab-anti-IGF-I), (1:3,000; 1:10,000), or rabbit serum (1:3,000; 1:10,000) as control.Gonadotropin-releasing hormone-induced LH release from primary cultures of male rat anterior pituitary cells.Insulin-like growth factor I induces a dose-dependent stimulus on the LH response to GnRH. Insulin-like growth factor I at doses of 10 and 15 nM exerted a maximal stimulus, increasing the LH response to GnRH by 30\%. The Ab-anti-IGF-I at both dilutions reduced the LH response to GnRH by 30\%. No effect was exerted by rabbit serum.Present data indicate that IGF-I may enhance the normal LH response to GnRH from rat anterior pituitary glands, suggesting important influences of this growth factor in the modulation of anterior pituitary LH release.

Modulation of anterior pituitary luteinizing hormone response to gonadotropin-releasing hormone by insulin-like growth factor I in vitro / R., Soldani; Cagnacci, Angelo; A. M., Paoletti; S. S., Yen; G. B., Melis. - In: FERTILITY AND STERILITY. - ISSN 0015-0282. - STAMPA. - 64:(1995), pp. 634-637.

Modulation of anterior pituitary luteinizing hormone response to gonadotropin-releasing hormone by insulin-like growth factor I in vitro.

CAGNACCI, Angelo;
1995

Abstract

To investigate how much insulin-like growth factor I may modulate the LH response to GnRH.In primary cultures of male rat anterior pituitary cells, the LH response to GnRH (10(-9) M) was evaluated after a 24-hour preincubation with medium alone, IGF-I (1, 5, 10, 15 nM), polyclonal antibody anti-IGF-I (Ab-anti-IGF-I), (1:3,000; 1:10,000), or rabbit serum (1:3,000; 1:10,000) as control.Gonadotropin-releasing hormone-induced LH release from primary cultures of male rat anterior pituitary cells.Insulin-like growth factor I induces a dose-dependent stimulus on the LH response to GnRH. Insulin-like growth factor I at doses of 10 and 15 nM exerted a maximal stimulus, increasing the LH response to GnRH by 30\%. The Ab-anti-IGF-I at both dilutions reduced the LH response to GnRH by 30\%. No effect was exerted by rabbit serum.Present data indicate that IGF-I may enhance the normal LH response to GnRH from rat anterior pituitary glands, suggesting important influences of this growth factor in the modulation of anterior pituitary LH release.
1995
64
634
637
Modulation of anterior pituitary luteinizing hormone response to gonadotropin-releasing hormone by insulin-like growth factor I in vitro / R., Soldani; Cagnacci, Angelo; A. M., Paoletti; S. S., Yen; G. B., Melis. - In: FERTILITY AND STERILITY. - ISSN 0015-0282. - STAMPA. - 64:(1995), pp. 634-637.
R., Soldani; Cagnacci, Angelo; A. M., Paoletti; S. S., Yen; G. B., Melis
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11380/740296
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