EnglishDexamethasone-receptor complexes can be extracted from HeLa cell nuclei by mild sonication. These complexes can account for 800-1000 binding sites/cell, and are indistinguishable from salt-extractable ones as judged by sucrose gradient centrifugation in the presence of 0.3 M KCl, showing a sedimentation coefficient of 3.6 S. These complexes, however, broadly sediment in the 7 to 3.6 S region of low salt sucrose gradients. Enzymatic treatment of soluble extracts from nuclear sonicates shows that RNA is associated to dexamethasone-receptor complexes.
RNase-sensitive glucocorticoid-receptor complexes from HeLa cell nuclei / Rossini, Gian Paolo; B., Barbiroli. - In: BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS. - ISSN 0006-291X. - STAMPA. - 113(1983), pp. 876-882.
| Data di pubblicazione: | 1983 |
| Titolo: | RNase-sensitive glucocorticoid-receptor complexes from HeLa cell nuclei |
| Autore/i: | Rossini, Gian Paolo; B., Barbiroli |
| Autore/i UNIMORE: | |
| Rivista: | |
| Volume: | 113 |
| Pagina iniziale: | 876 |
| Pagina finale: | 882 |
| Codice identificativo ISI: | WOS:A1983QX60100021 |
| Codice identificativo Scopus: | 2-s2.0-0020583787 |
| Codice identificativo Pubmed: | 6870897 |
| Citazione: | RNase-sensitive glucocorticoid-receptor complexes from HeLa cell nuclei / Rossini, Gian Paolo; B., Barbiroli. - In: BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS. - ISSN 0006-291X. - STAMPA. - 113(1983), pp. 876-882. |
| Tipologia | Articolo su rivista |
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