The metabolic profile of human healthy and neoplastic colorectal tissues was obtained using ex vivo High-Resolution Magic Angle Spinning (HR-MAS) NMR spectroscopy. Principal Components Analysis (PCA) and Partial Least Squares Discriminant Analysis (PLS-DA) were applied to NMR data in order to highlight the biochemical differences between healthy and neoplastic colorectal tissues. The synergic combination of ex vivo HR-MAS NMR spectroscopy with Multivariate Data Analysis enables discrimination between healthy and tumoral colorectal tissues and identification of the increase of taurine, acetate, lactate, and lipids, and the decrease of polyols and sugars as tumoral characteristics. Moreover, it was found that macroscopically/histologically normal colorectal tissues, collected at least 15 cm from the adenocarcinoma, are characterized by a metabolic pattern quite similar to that typical of tumoral lesions.It was shown that ex vivo HR-MAS NMR spectroscopy, performed on intact specimens, may be of great potentiality in the clinical evaluation of human neoplastic colorectal tissues and that thebiochemical data represent the molecular basis for an accurate and noninvasive clinical applications of in vivo NMR spectroscopy.
|Data di pubblicazione:||2009|
|Titolo:||Discrimination of Healthy and Neoplastic Human Colon Tissues by ex Vivo HR-MAS NMR Spectroscopy and Chemometric Analyses|
|Autori:||V. Righi; C. Durante; M. Cocchi; C. Calabrese; G. Di Febo; F. Lecce; A. Pisi; V. Tugnoli; A. Mucci; L. Schenetti|
|Digital Object Identifier (DOI):||10.1021/pr801094b|
|Appare nelle tipologie:||Articolo su rivista|
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