A pool of new Zygosaccharomyces strains was isolated in Traditional Balsamic Vinegar samples. Recently an our multi-genestudy [including genes of the rDNA repeat and two nuclear genes, SOD2 and HIS3] has highlighted that: i) rDNA units areunusually polymorphic with respect to ITS regions (with three highly divergent ITS sequences), but the D1/D2 26S rDNA regionis unique and monomorphic; ii) two different copies of nuclear genes SOD2 and HIS3 are present. In this study a physiologicaland genotypic characterization of two of these strains, namely ABT301 and ABT601, was performed. Physiological tests showedthat these strains are osmophilic and halophilic yeasts able to grow at 60% glucose and 16% NaCl and with a assimilative profilepartially similar to that of Z. rouxii species. Chromosomal DNA separation by PFEG was performed in order estimate the numberand size of their chromosomes. A SOD2-22-based Real Time PCR assay was designed to determine the ploidy of ABT301 andABT601, compared to diploid Z. rouxii CBS732 type strain. Finally three transcriptional assays were carried out: the first wasaimed to 5.8S-ITS region of 35S precursor rRNA, the others to the two different SOD2 and HIS3 copies. According to ourpreliminary results, ABT301 and ABT601 showed: i) a different electrophoretic karyotype compared to CBS732T strain; ii) adiploid status; iii) both copies of SOD2 and HIS3 genes actively transcribed, as well as the three 5.8S-ITS regions.
Physiological and genotypic characterization of a novel Zygosaccharomyces species / Solieri, Lisa; Cassanelli, Stefano; Giudici, Paolo. - ELETTRONICO. - 24:(2007), pp. S40-S40. (Intervento presentato al convegno XXIIIrd International Conference on Yeast Genetics and Molecular Biology tenutosi a Melbourne, Australia nel 1-6 july 2007).
Physiological and genotypic characterization of a novel Zygosaccharomyces species
SOLIERI, lisa;CASSANELLI, Stefano;GIUDICI, Paolo
2007
Abstract
A pool of new Zygosaccharomyces strains was isolated in Traditional Balsamic Vinegar samples. Recently an our multi-genestudy [including genes of the rDNA repeat and two nuclear genes, SOD2 and HIS3] has highlighted that: i) rDNA units areunusually polymorphic with respect to ITS regions (with three highly divergent ITS sequences), but the D1/D2 26S rDNA regionis unique and monomorphic; ii) two different copies of nuclear genes SOD2 and HIS3 are present. In this study a physiologicaland genotypic characterization of two of these strains, namely ABT301 and ABT601, was performed. Physiological tests showedthat these strains are osmophilic and halophilic yeasts able to grow at 60% glucose and 16% NaCl and with a assimilative profilepartially similar to that of Z. rouxii species. Chromosomal DNA separation by PFEG was performed in order estimate the numberand size of their chromosomes. A SOD2-22-based Real Time PCR assay was designed to determine the ploidy of ABT301 andABT601, compared to diploid Z. rouxii CBS732 type strain. Finally three transcriptional assays were carried out: the first wasaimed to 5.8S-ITS region of 35S precursor rRNA, the others to the two different SOD2 and HIS3 copies. According to ourpreliminary results, ABT301 and ABT601 showed: i) a different electrophoretic karyotype compared to CBS732T strain; ii) adiploid status; iii) both copies of SOD2 and HIS3 genes actively transcribed, as well as the three 5.8S-ITS regions.
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