Since PHA-stimulated active T lymphocytes from aged humans showed changes in the metabolic pattern of inositol lipids in comparison with young subjects, we studied the possible role of phosphoinositidase C (PIC) in the generation of this phenomenon. The breakdown of exogenous [3H]phosphatidylinositol 4,5-bisphosphate was found to be optimal at neutral pH and Ca++ concentrations close to millimolar levels. Under these conditions PIC activity of resting lymphocytes did not differ in aged and young subjects, while, after short periods of PHA stimulation (up to 4 hr) the substrate hydrolysis was lower and delayed in the elderly group in comparison with that of controls. Our findings support the hypothesis that the age-related default of this enzyme, responsible for the age-related changes in the inositol lipid pathway of this peculiar subpopulation, could be involved, as a primary event, in the mechanisms leading to the reduced proliferative response of aged active T lymphocytes. IF 2.9476
|Anno di pubblicazione:||1993|
|Titolo:||Age-related events in human active T limphocytes. Changes in the phosphoinositidase C activity.|
|Autori:||R. DI PIETRO; R.A. RANA; A. SCISCIO; S. MARMIROLI; A.M. BILLI; A. CATALDI; L. COCCO.|
|Appare nelle tipologie:||Articolo su rivista|
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