The intrinsic steady-state and time-resolved fluorescence of Leishmania major pteridine reductase 1, a tetrameric protein target for anti-infective agents, is investigated and deciphered in terms of the contributions from populations of the two tryptophans included in each protein monomer. Signals from these local fluorometric reporters contain molecular-level information on the conformational landscape of this protein and on its interaction with a nanomolar pteridinic inhibitor
Intrinsic Fluorometric Reporters of Pteridine Reductase 1, a Target for Antiparasitic Agents / Ferrari, S.; Costi, M. P.; Ponterini, G.. - In: PHYSCHEM. - ISSN 2673-7167. - 2:2(2022), pp. 131-144. [10.3390/physchem2020010]
Intrinsic Fluorometric Reporters of Pteridine Reductase 1, a Target for Antiparasitic Agents
M. P. Costi;G. Ponterini
2022
Abstract
The intrinsic steady-state and time-resolved fluorescence of Leishmania major pteridine reductase 1, a tetrameric protein target for anti-infective agents, is investigated and deciphered in terms of the contributions from populations of the two tryptophans included in each protein monomer. Signals from these local fluorometric reporters contain molecular-level information on the conformational landscape of this protein and on its interaction with a nanomolar pteridinic inhibitor
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