The intrinsic steady-state and time-resolved fluorescence of Leishmania major pteridine reductase 1, a tetrameric protein target for anti-infective agents, is investigated and deciphered in terms of the contributions from populations of the two tryptophans included in each protein monomer. Signals from these local fluorometric reporters contain molecular-level information on the conformational landscape of this protein and on its interaction with a nanomolar pteridinic inhibitor
Intrinsic Fluorometric Reporters of Pteridine Reductase 1, a Target for Antiparasitic Agents / Ferrari, S.; Costi, M. P.; Ponterini, G.. - In: PHYSCHEM. - ISSN 2673-7167. - 2:(2022), pp. 131-144. [10.3390/physchem2020010]
Intrinsic Fluorometric Reporters of Pteridine Reductase 1, a Target for Antiparasitic Agents
M. P. Costi;G. Ponterini
2022
Abstract
The intrinsic steady-state and time-resolved fluorescence of Leishmania major pteridine reductase 1, a tetrameric protein target for anti-infective agents, is investigated and deciphered in terms of the contributions from populations of the two tryptophans included in each protein monomer. Signals from these local fluorometric reporters contain molecular-level information on the conformational landscape of this protein and on its interaction with a nanomolar pteridinic inhibitor
| File | Dimensione | Formato | |
|---|---|---|---|
|
fluo PTR1 physchem-02-00010.pdf
Open access
Descrizione: paper text
Tipologia:
Versione pubblicata dall'editore
Dimensione
3.5 MB
Formato
Adobe PDF
|
3.5 MB | Adobe PDF | Visualizza/Apri |
Pubblicazioni consigliate
I metadati presenti in IRIS UNIMORE sono rilasciati con licenza Creative Commons CC0 1.0 Universal, mentre i file delle pubblicazioni sono rilasciati con licenza Attribuzione 4.0 Internazionale (CC BY 4.0), salvo diversa indicazione.
In caso di violazione di copyright, contattare Supporto Iris
