Urinary proteomics is primarily applied to the study of renal and urogenital tract disorders. Here are reported two distinct successful examples of this approach for the discovery of early urinary biomarkers of kidney related dysfunctions: diabetic nephropathy (DN), a well known complication of diabetes frequently leading to dialysis, and druginduced nephrotoxicity, a possible condition caused by medication overuse headache (MOH). Early detection of kidney disorders based on selective biomarkers could permit to diagnose patients at the initial stage of the disease, where the therapy is still possible to stop or prevent occurrence of advance disease. Urine samples were first concentrated and desalted. Subsequently, they were subjected to two-dimensional gel electrophoresis (2DE) coupled to mass spectrometry (MS) for protein identification. Furthermore, some proteins were verified by Western blot and ELISA test. In diabetes-related study, 11 differentially expressed proteins were detected (8 upregulated and 3 downregulated) in type 2 diabetic (T2D) and T2DN patients compared to the healthy control subjects. In MOH study, a total of 21 overexcreted proteins was revealed in urine of non-steroidal antiinflammatory drugs (NSAIDs) and mixtures abusers vs controls. Particularly, 4 proteins were positively validated by immunoblotting and ELISA. Urinary proteomics allows noninvasive assessment of renal diseases at an early stage by the identification of characteristic protein pattern.
Diagnostic proteomic biomarkers to detect kidney diseases / Ozben, Tomris; Bellei, Elisa; Monari, Emanuela; Bergamini, Stefania; Pini, Luigi Alberto; Tomasi, Aldo. - In: FEBS OPEN BIO. - ISSN 2211-5463. - 11:Suppl. 1(2021), pp. 39-40. (Intervento presentato al convegno The 45TH FEBS CONGRESS; Molecules of Life: Towards New Horizons. tenutosi a Ljubljana, Slovenia nel 3-8 July, 2021) [10.1002/2211-5463.13206].
Diagnostic proteomic biomarkers to detect kidney diseases
Bellei Elisa;Monari Emanuela;Bergamini Stefania;Pini Luigi Alberto;Tomasi Aldo
2021
Abstract
Urinary proteomics is primarily applied to the study of renal and urogenital tract disorders. Here are reported two distinct successful examples of this approach for the discovery of early urinary biomarkers of kidney related dysfunctions: diabetic nephropathy (DN), a well known complication of diabetes frequently leading to dialysis, and druginduced nephrotoxicity, a possible condition caused by medication overuse headache (MOH). Early detection of kidney disorders based on selective biomarkers could permit to diagnose patients at the initial stage of the disease, where the therapy is still possible to stop or prevent occurrence of advance disease. Urine samples were first concentrated and desalted. Subsequently, they were subjected to two-dimensional gel electrophoresis (2DE) coupled to mass spectrometry (MS) for protein identification. Furthermore, some proteins were verified by Western blot and ELISA test. In diabetes-related study, 11 differentially expressed proteins were detected (8 upregulated and 3 downregulated) in type 2 diabetic (T2D) and T2DN patients compared to the healthy control subjects. In MOH study, a total of 21 overexcreted proteins was revealed in urine of non-steroidal antiinflammatory drugs (NSAIDs) and mixtures abusers vs controls. Particularly, 4 proteins were positively validated by immunoblotting and ELISA. Urinary proteomics allows noninvasive assessment of renal diseases at an early stage by the identification of characteristic protein pattern.File | Dimensione | Formato | |
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