The scientific interest in Cannabis has been documented by a wide literature but postmortem studies and interpretations of autopsy findings are lacking or limited to few cases, few matrices analyzed or a small number of analytes. The present study describes the development and full in-house validation of a sensitive and simple method based on an optimized rapid clean up procedure combined with a robust and highly sensitive LC-MS-MS technique, designed to simultaneous determination of Delta9-tetrahydrocannabinol (THC), cannabidiol (CBD), 11-hydroxy-Delta9-tetrahydrocannabinol (11-OH-THC), 11-nor-Delta9-tetrahydrocannabinol-carboxylic acid (THC-COOH) and 11-nor-Delta9-tetrahydrocannabinol-carboxylic acid-glucuronated (THC-COOH-glucuronide) in postmortem samples: central blood (CB), femoral blood (FB) and brain tissue (BR). The developed method was validated and applied to 24 postmortem cases involving cannabinoids. In this study, we presented a full optimization and validation of target analyses for each matrix. The procedure had proven to be reliable and accurate. This study adds new data, particularly about the cannabinoids concentrations in BR samples. Combined pattern (CB, FB, BR) can be used in the interpretation of postmortem cases, proving and strengthening the assessments made on blood data. BR matrix in a helpful supplement in the investigation of the role of cannabinoids as crucial or contributory factor in leading to death.

Cannabinoids Determination in Brain: A Supplemental Helpful in Postmortem Evaluation / Palazzoli, F; Santunione, A L; Marchesi, F; Verri, P; Vandelli, D; Licata, M; Silingardi, E. - In: JOURNAL OF ANALYTICAL TOXICOLOGY. - ISSN 0146-4760. - 45:9(2020), pp. 918-926. [10.1093/jat/bkaa155]

Cannabinoids Determination in Brain: A Supplemental Helpful in Postmortem Evaluation

Palazzoli, F
;
Santunione, A L;Verri, P;Vandelli, D;Licata, M;Silingardi, E
2020-01-01

Abstract

The scientific interest in Cannabis has been documented by a wide literature but postmortem studies and interpretations of autopsy findings are lacking or limited to few cases, few matrices analyzed or a small number of analytes. The present study describes the development and full in-house validation of a sensitive and simple method based on an optimized rapid clean up procedure combined with a robust and highly sensitive LC-MS-MS technique, designed to simultaneous determination of Delta9-tetrahydrocannabinol (THC), cannabidiol (CBD), 11-hydroxy-Delta9-tetrahydrocannabinol (11-OH-THC), 11-nor-Delta9-tetrahydrocannabinol-carboxylic acid (THC-COOH) and 11-nor-Delta9-tetrahydrocannabinol-carboxylic acid-glucuronated (THC-COOH-glucuronide) in postmortem samples: central blood (CB), femoral blood (FB) and brain tissue (BR). The developed method was validated and applied to 24 postmortem cases involving cannabinoids. In this study, we presented a full optimization and validation of target analyses for each matrix. The procedure had proven to be reliable and accurate. This study adds new data, particularly about the cannabinoids concentrations in BR samples. Combined pattern (CB, FB, BR) can be used in the interpretation of postmortem cases, proving and strengthening the assessments made on blood data. BR matrix in a helpful supplement in the investigation of the role of cannabinoids as crucial or contributory factor in leading to death.
8-ott-2020
45
9
918
926
Cannabinoids Determination in Brain: A Supplemental Helpful in Postmortem Evaluation / Palazzoli, F; Santunione, A L; Marchesi, F; Verri, P; Vandelli, D; Licata, M; Silingardi, E. - In: JOURNAL OF ANALYTICAL TOXICOLOGY. - ISSN 0146-4760. - 45:9(2020), pp. 918-926. [10.1093/jat/bkaa155]
Palazzoli, F; Santunione, A L; Marchesi, F; Verri, P; Vandelli, D; Licata, M; Silingardi, E
File in questo prodotto:
File Dimensione Formato  
Cannabinoids Determination in Brain.pdf

Accesso riservato

Descrizione: articolo principale
Tipologia: Versione pubblicata dall'editore
Dimensione 152.46 kB
Formato Adobe PDF
152.46 kB Adobe PDF   Visualizza/Apri   Richiedi una copia
Pubblicazioni consigliate

Licenza Creative Commons
I metadati presenti in IRIS UNIMORE sono rilasciati con licenza Creative Commons CC0 1.0 Universal, mentre i file delle pubblicazioni sono rilasciati con licenza Attribuzione 4.0 Internazionale (CC BY 4.0), salvo diversa indicazione.
In caso di violazione di copyright, contattare Supporto Iris

Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11380/1244775
Citazioni
  • ???jsp.display-item.citation.pmc??? 1
  • Scopus 1
  • ???jsp.display-item.citation.isi??? 1
social impact