Successful treatment of Acinetobacter baumannii infections require early and appropriate antimicrobial therapy. One of the first steps in this process is understanding which β-lactamase (bla) alleles are present and in what combinations. Thus, we performed WGS on 98 carbapenem-resistant A. baumannii (CR Ab). In most isolates, an acquired blaOXA carbapenemase was found in addition to the intrinsic blaOXA allele. The most commonly found allele was blaOXA-23 (n = 78/98). In some isolates, blaOXA-23 was found in addition to other carbapenemase alleles: blaOXA-82 (n = 12/78), blaOXA-72 (n = 2/78) and blaOXA-24/40 (n = 1/78). Surprisingly, 20% of isolates carried carbapenemases not routinely assayed for by rapid molecular diagnostic platforms, i.e., blaOXA-82 and blaOXA-172; all had ISAba1 elements. In 8 CR Ab, blaOXA-82 or blaOXA-172 was the only carbapenemase. Both blaOXA-24/40 and its variant blaOXA-72 were each found in 6/98 isolates. The most prevalent ADC variants were blaADC-30 (21%), blaADC-162 (21%), and blaADC-212 (26%). Complete combinations are reported.

A comprehensive and contemporary “snapshot” of β-lactamases in carbapenem resistant Acinetobacter baumannii / Hujer, A. M.; Hujer, K. M.; Leonard, D. A.; Powers, R. A.; Wallar, B. J.; Mack, A. R.; Taracila, M. A.; Rather, P. N.; Higgins, P. G.; Prati, F.; Caselli, E.; Marshall, S. H.; Clarke, T.; Greco, C.; Venepally, P.; Brinkac, L.; Kreiswirth, B. N.; Fouts, D. E.; Bonomo, R. A.. - In: DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE. - ISSN 0732-8893. - 99:2(2021), pp. 1-6. [10.1016/j.diagmicrobio.2020.115242]

A comprehensive and contemporary “snapshot” of β-lactamases in carbapenem resistant Acinetobacter baumannii

Prati F.;Caselli E.;
2021

Abstract

Successful treatment of Acinetobacter baumannii infections require early and appropriate antimicrobial therapy. One of the first steps in this process is understanding which β-lactamase (bla) alleles are present and in what combinations. Thus, we performed WGS on 98 carbapenem-resistant A. baumannii (CR Ab). In most isolates, an acquired blaOXA carbapenemase was found in addition to the intrinsic blaOXA allele. The most commonly found allele was blaOXA-23 (n = 78/98). In some isolates, blaOXA-23 was found in addition to other carbapenemase alleles: blaOXA-82 (n = 12/78), blaOXA-72 (n = 2/78) and blaOXA-24/40 (n = 1/78). Surprisingly, 20% of isolates carried carbapenemases not routinely assayed for by rapid molecular diagnostic platforms, i.e., blaOXA-82 and blaOXA-172; all had ISAba1 elements. In 8 CR Ab, blaOXA-82 or blaOXA-172 was the only carbapenemase. Both blaOXA-24/40 and its variant blaOXA-72 were each found in 6/98 isolates. The most prevalent ADC variants were blaADC-30 (21%), blaADC-162 (21%), and blaADC-212 (26%). Complete combinations are reported.
2021
99
2
1
6
A comprehensive and contemporary “snapshot” of β-lactamases in carbapenem resistant Acinetobacter baumannii / Hujer, A. M.; Hujer, K. M.; Leonard, D. A.; Powers, R. A.; Wallar, B. J.; Mack, A. R.; Taracila, M. A.; Rather, P. N.; Higgins, P. G.; Prati, F.; Caselli, E.; Marshall, S. H.; Clarke, T.; Greco, C.; Venepally, P.; Brinkac, L.; Kreiswirth, B. N.; Fouts, D. E.; Bonomo, R. A.. - In: DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE. - ISSN 0732-8893. - 99:2(2021), pp. 1-6. [10.1016/j.diagmicrobio.2020.115242]
Hujer, A. M.; Hujer, K. M.; Leonard, D. A.; Powers, R. A.; Wallar, B. J.; Mack, A. R.; Taracila, M. A.; Rather, P. N.; Higgins, P. G.; Prati, F.; Caselli, E.; Marshall, S. H.; Clarke, T.; Greco, C.; Venepally, P.; Brinkac, L.; Kreiswirth, B. N.; Fouts, D. E.; Bonomo, R. A.
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