We report here on a reflection-type long period fiber grating (RT-LPG) biosensor for the fast detection of class C (AmpC) β-lactamases (BLs), actually considered as one of the most important source of resistance to β-lactam antibiotics expressed by resistant bacteria. A standard LPG working in transmission configuration is first transformed in a more practical probe working in reflection mode and successively coated with a primary high refractive index (HRI) overlay of atactic polystyrene (aPS) in order to increase its surrounding refractive index sensitivity (SRI) in biological solutions. The aPS-coated RT-LPG is then coated by a secondary layer of poly(methylmethacrylate)-co-methacrylic acid (PMMA-co-MA) in order to provide the necessary surface functionalities to promote a stable covalent bioreceptors immobilization. The BLs detection has been performed by using the 3-aminophenylboronic acid (3-APBA) as biorecognition element, due to its excellent inhibition properties against class C BLs and specificity. Results here provided demonstrate that the proposed label free biosensor is capable of reliable detection of purified AmpC BLs in phosphate buffer solutions (PBS) with concentrations as low as one hundred nM, with a lowest limit of detection (LOD) of the order of a few tens of nM. The real effectiveness of the proposed biosensor has been also confirmed in lysate samples, which contain Escherichia coli bacteria overexpressing AmpC BLs.

Long period fiber grating working in reflection mode as valuable biosensing platform for the detection of drug resistant bacteria / Quero, G.; Zuppolini, S.; Consales, M.; Diodato, L.; Vaiano, P.; Venturelli, A.; Santucci, M.; Spyrakis, F.; Costi, M. P.; Giordano, M.; Borriello, A.; Cutolo, A.; Cusano, A.. - In: SENSORS AND ACTUATORS. B, CHEMICAL. - ISSN 0925-4005. - 230:(2016), pp. 510-520. [10.1016/j.snb.2016.02.086]

Long period fiber grating working in reflection mode as valuable biosensing platform for the detection of drug resistant bacteria

Venturelli A.;Santucci M.
Methodology
;
Spyrakis F.
Methodology
;
Costi M. P.
Conceptualization
;
2016-01-01

Abstract

We report here on a reflection-type long period fiber grating (RT-LPG) biosensor for the fast detection of class C (AmpC) β-lactamases (BLs), actually considered as one of the most important source of resistance to β-lactam antibiotics expressed by resistant bacteria. A standard LPG working in transmission configuration is first transformed in a more practical probe working in reflection mode and successively coated with a primary high refractive index (HRI) overlay of atactic polystyrene (aPS) in order to increase its surrounding refractive index sensitivity (SRI) in biological solutions. The aPS-coated RT-LPG is then coated by a secondary layer of poly(methylmethacrylate)-co-methacrylic acid (PMMA-co-MA) in order to provide the necessary surface functionalities to promote a stable covalent bioreceptors immobilization. The BLs detection has been performed by using the 3-aminophenylboronic acid (3-APBA) as biorecognition element, due to its excellent inhibition properties against class C BLs and specificity. Results here provided demonstrate that the proposed label free biosensor is capable of reliable detection of purified AmpC BLs in phosphate buffer solutions (PBS) with concentrations as low as one hundred nM, with a lowest limit of detection (LOD) of the order of a few tens of nM. The real effectiveness of the proposed biosensor has been also confirmed in lysate samples, which contain Escherichia coli bacteria overexpressing AmpC BLs.
230
510
520
Long period fiber grating working in reflection mode as valuable biosensing platform for the detection of drug resistant bacteria / Quero, G.; Zuppolini, S.; Consales, M.; Diodato, L.; Vaiano, P.; Venturelli, A.; Santucci, M.; Spyrakis, F.; Costi, M. P.; Giordano, M.; Borriello, A.; Cutolo, A.; Cusano, A.. - In: SENSORS AND ACTUATORS. B, CHEMICAL. - ISSN 0925-4005. - 230:(2016), pp. 510-520. [10.1016/j.snb.2016.02.086]
Quero, G.; Zuppolini, S.; Consales, M.; Diodato, L.; Vaiano, P.; Venturelli, A.; Santucci, M.; Spyrakis, F.; Costi, M. P.; Giordano, M.; Borriello, A.; Cutolo, A.; Cusano, A.
File in questo prodotto:
File Dimensione Formato  
Quero-SAB-2016-preprint.doc

Accesso riservato

Tipologia: Versione originale dell'autore proposta per la pubblicazione
Dimensione 2.54 MB
Formato Microsoft Word
2.54 MB Microsoft Word   Visualizza/Apri   Richiedi una copia
quero2016.pdf

Accesso riservato

Tipologia: Versione pubblicata dall'editore
Dimensione 2.3 MB
Formato Adobe PDF
2.3 MB Adobe PDF   Visualizza/Apri   Richiedi una copia
Pubblicazioni consigliate

Licenza Creative Commons
I metadati presenti in IRIS UNIMORE sono rilasciati con licenza Creative Commons CC0 1.0 Universal, mentre i file delle pubblicazioni sono rilasciati con licenza Attribuzione 4.0 Internazionale (CC BY 4.0), salvo diversa indicazione.
In caso di violazione di copyright, contattare Supporto Iris

Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11380/1208952
Citazioni
  • ???jsp.display-item.citation.pmc??? ND
  • Scopus 36
  • ???jsp.display-item.citation.isi??? 31
social impact