Samples (n = 485) of raw (n = 394) or heat-treated (n = 91) milk of three different species (cow, n = 170; sheep, n = 133; donkey, n = 84), collected 2013–2016 in Western Sicily (Southern Italy), were analyzed for aflatoxin M1 (AFM1) by enzyme-linked immunosorbent assay (ELISA). Positive ELISA results were further analyzed by HPLC with fluorescence detection. Both methods had a detection limit for AFM1 in milk of 7 ng kg−1. ELISA yielded 12.9 and 5% positives in cows and sheep milk, respectively, all samples of donkey milk were negative. Levels of AFM1 were in most cases at 0.007–< 0.05 μg kg−1, only two samples (sheep milk) slightly exceeded the European Union maximum level of 0.05 μg kg−1. Only 6% of the samples were positive for AFM1 in a concentration range of 0.008–0.15 μg kg−1. Only milk samples collected directly from farms were positive. Overall, the levels were much lower than previously reported for Southern Italy cow and sheep milk samples purchased in retail stores. The results of this work indicate a continuous improvement of the feeding techniques on dairy farms of Southern Italy, which is essential to ensure consumers’ protection.

Aflatoxin M1 in cow, sheep, and donkey milk produced in Sicily, Southern Italy / Cammilleri, Gaetano; Graci, Stefania; Collura, Rosaria; Buscemi, Maria Drussilla; Vella, Antonio; Macaluso, Andrea; Giaccone, Vita; Giangrosso, Giuseppe; Cicero, Antonello; Lo Dico, Gianluigi Maria; Pulvirenti, Andrea; Cicero, Nicola; Ferrantelli, Vincenzo. - In: MYCOTOXIN RESEARCH. - ISSN 0178-7888. - 35:(2019), pp. 47-53. [10.1007/s12550-018-0329-y]

Aflatoxin M1 in cow, sheep, and donkey milk produced in Sicily, Southern Italy

Cammilleri, Gaetano
;
Vella, Antonio;Macaluso, Andrea;Pulvirenti, Andrea
Membro del Collaboration Group
;
2019

Abstract

Samples (n = 485) of raw (n = 394) or heat-treated (n = 91) milk of three different species (cow, n = 170; sheep, n = 133; donkey, n = 84), collected 2013–2016 in Western Sicily (Southern Italy), were analyzed for aflatoxin M1 (AFM1) by enzyme-linked immunosorbent assay (ELISA). Positive ELISA results were further analyzed by HPLC with fluorescence detection. Both methods had a detection limit for AFM1 in milk of 7 ng kg−1. ELISA yielded 12.9 and 5% positives in cows and sheep milk, respectively, all samples of donkey milk were negative. Levels of AFM1 were in most cases at 0.007–< 0.05 μg kg−1, only two samples (sheep milk) slightly exceeded the European Union maximum level of 0.05 μg kg−1. Only 6% of the samples were positive for AFM1 in a concentration range of 0.008–0.15 μg kg−1. Only milk samples collected directly from farms were positive. Overall, the levels were much lower than previously reported for Southern Italy cow and sheep milk samples purchased in retail stores. The results of this work indicate a continuous improvement of the feeding techniques on dairy farms of Southern Italy, which is essential to ensure consumers’ protection.
2019
13-set-2018
35
47
53
Aflatoxin M1 in cow, sheep, and donkey milk produced in Sicily, Southern Italy / Cammilleri, Gaetano; Graci, Stefania; Collura, Rosaria; Buscemi, Maria Drussilla; Vella, Antonio; Macaluso, Andrea; Giaccone, Vita; Giangrosso, Giuseppe; Cicero, Antonello; Lo Dico, Gianluigi Maria; Pulvirenti, Andrea; Cicero, Nicola; Ferrantelli, Vincenzo. - In: MYCOTOXIN RESEARCH. - ISSN 0178-7888. - 35:(2019), pp. 47-53. [10.1007/s12550-018-0329-y]
Cammilleri, Gaetano; Graci, Stefania; Collura, Rosaria; Buscemi, Maria Drussilla; Vella, Antonio; Macaluso, Andrea; Giaccone, Vita; Giangrosso, Giuseppe; Cicero, Antonello; Lo Dico, Gianluigi Maria; Pulvirenti, Andrea; Cicero, Nicola; Ferrantelli, Vincenzo
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11380/1165399
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