This paper reports the experimental assessment of an automated optical assay based on label free optical fiber optrodes for the fast detection of class C beta-lactamases (AmpC BLs), actually considered as one of the most important sources of resistance to beta-lactams antibiotics expressed by resistant bacteria. Reflection-type long period fiber gratings (RT-LPG) have been used as highly sensitive label free optrodes, while a higher affine boronic acid based ligand was here selected to enhance the overall assay performances compared to those obtained in our first demonstration. In order to prove the feasibility analysis towards a fully automated optical assay, an engineered system was developed to simultaneously manipulate and interrogate multiple fiber optic optrodes in the different phases of the assay. The automated system tested in AmpC solutions at increasing concentrations demonstrated a limit of detection (LOD) of 6 nM, three times better when compared with the results obtained in our previous work. Moreover, the real effectiveness of the proposed optical assay has been also confirmed in complex matrices as the case of lysates of Escherichia coli overexpressing AmpC. (C) 2017 Optical Society of America
Label-free fiber optic optrode for the detection of class C beta-lactamases expressed by drug resistant bacteria / Zuppolini, S; Quero, G; Consales, M; Diodato, L; Vaiano, P; Venturelli, A; Santucci, M; Spyrakis, F; Costi, Mp; Giordano, M; Cutolo, A; Cusano, A; Borriello, Alessandro. - In: BIOMEDICAL OPTICS EXPRESS. - ISSN 2156-7085. - 8:11(2017), pp. 5191-5205. [10.1364/BOE.8.005191]
Label-free fiber optic optrode for the detection of class C beta-lactamases expressed by drug resistant bacteria
Venturelli, A;Santucci, M;Spyrakis, F;Costi, MP;BORRIELLO, ALESSANDRO
2017
Abstract
This paper reports the experimental assessment of an automated optical assay based on label free optical fiber optrodes for the fast detection of class C beta-lactamases (AmpC BLs), actually considered as one of the most important sources of resistance to beta-lactams antibiotics expressed by resistant bacteria. Reflection-type long period fiber gratings (RT-LPG) have been used as highly sensitive label free optrodes, while a higher affine boronic acid based ligand was here selected to enhance the overall assay performances compared to those obtained in our first demonstration. In order to prove the feasibility analysis towards a fully automated optical assay, an engineered system was developed to simultaneously manipulate and interrogate multiple fiber optic optrodes in the different phases of the assay. The automated system tested in AmpC solutions at increasing concentrations demonstrated a limit of detection (LOD) of 6 nM, three times better when compared with the results obtained in our previous work. Moreover, the real effectiveness of the proposed optical assay has been also confirmed in complex matrices as the case of lysates of Escherichia coli overexpressing AmpC. (C) 2017 Optical Society of AmericaFile | Dimensione | Formato | |
---|---|---|---|
BOE_2017.pdf
Open access
Descrizione: Articolo principale
Tipologia:
Versione pubblicata dall'editore
Dimensione
3.66 MB
Formato
Adobe PDF
|
3.66 MB | Adobe PDF | Visualizza/Apri |
Pubblicazioni consigliate
I metadati presenti in IRIS UNIMORE sono rilasciati con licenza Creative Commons CC0 1.0 Universal, mentre i file delle pubblicazioni sono rilasciati con licenza Attribuzione 4.0 Internazionale (CC BY 4.0), salvo diversa indicazione.
In caso di violazione di copyright, contattare Supporto Iris