Functional properties of cell membranes depend on their composition, particularly on the relative amount of saturated, unsaturated and polyunsaturated fatty acids present in the phospholipids. The aim of this study was to investigate the effect of cell membrane composition on cell fitness, adaptation and stress response in Kluyveromyces lactis. To this purpose, we have deleted the genes FAD2 and FAD3 encoding Δ12 and ω3 desaturases in Kluyveromyces lactis, thus generating mutant strains with altered fatty acid composition of membranes. These strains were viable and able to grow in stressing conditions like hypoxia and low temperature. Deletion of the Δ9 desaturase-encoding gene KlOLE1 resulted in lethality, suggesting that this enzyme has an essential role in this yeast. Transcription of the desaturase genes KlOLE1, FAD2 and FAD3 and cellular localization of the corresponding enzymes, have been studied under hypoxia and cold stress. Our findings indicate that expression of these desaturase genes and membrane composition were modulated by hypoxia and temperature stress, although the changes induced by these and other assayed conditions did not dramatically affect the general cellular fitness.

Functional roles of the fatty acid desaturases encoded by KLOLE1, FAD2 and FAD3 in the yeast Kluyveromyces lactis / de Angelis, Lorenzo; Rinaldi, Teresa; Cirigliano, Angela; Bello, Cristiano; Reverberi, Massimo; Amaretti, Alberto; Montanari, Arianna; Santomartino, Rosa; Raimondi, Stefano; Gonzalez, Alicia; Bianchi, Michele M.. - In: MICROBIOLOGY. - ISSN 1350-0872. - STAMPA. - 162:(2016), pp. 1435-1445. [10.1099/mic.0.000315]

Functional roles of the fatty acid desaturases encoded by KLOLE1, FAD2 and FAD3 in the yeast Kluyveromyces lactis

AMARETTI, Alberto;RAIMONDI, Stefano;
2016

Abstract

Functional properties of cell membranes depend on their composition, particularly on the relative amount of saturated, unsaturated and polyunsaturated fatty acids present in the phospholipids. The aim of this study was to investigate the effect of cell membrane composition on cell fitness, adaptation and stress response in Kluyveromyces lactis. To this purpose, we have deleted the genes FAD2 and FAD3 encoding Δ12 and ω3 desaturases in Kluyveromyces lactis, thus generating mutant strains with altered fatty acid composition of membranes. These strains were viable and able to grow in stressing conditions like hypoxia and low temperature. Deletion of the Δ9 desaturase-encoding gene KlOLE1 resulted in lethality, suggesting that this enzyme has an essential role in this yeast. Transcription of the desaturase genes KlOLE1, FAD2 and FAD3 and cellular localization of the corresponding enzymes, have been studied under hypoxia and cold stress. Our findings indicate that expression of these desaturase genes and membrane composition were modulated by hypoxia and temperature stress, although the changes induced by these and other assayed conditions did not dramatically affect the general cellular fitness.
27-mag-2016
162
1435
1445
Functional roles of the fatty acid desaturases encoded by KLOLE1, FAD2 and FAD3 in the yeast Kluyveromyces lactis / de Angelis, Lorenzo; Rinaldi, Teresa; Cirigliano, Angela; Bello, Cristiano; Reverberi, Massimo; Amaretti, Alberto; Montanari, Arianna; Santomartino, Rosa; Raimondi, Stefano; Gonzalez, Alicia; Bianchi, Michele M.. - In: MICROBIOLOGY. - ISSN 1350-0872. - STAMPA. - 162:(2016), pp. 1435-1445. [10.1099/mic.0.000315]
de Angelis, Lorenzo; Rinaldi, Teresa; Cirigliano, Angela; Bello, Cristiano; Reverberi, Massimo; Amaretti, Alberto; Montanari, Arianna; Santomartino, Rosa; Raimondi, Stefano; Gonzalez, Alicia; Bianchi, Michele M.
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Utilizza questo identificativo per citare o creare un link a questo documento: http://hdl.handle.net/11380/1111418
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