The pH-dependence of the reduction potential determined through differential pulse voltammetry for the high potential iron sulfur proteins (HiPIP) from R. globiformis, C. vinosum, R. gelatinosus, E. vacuolata (I and II), E. halophila (I and II) is reported. A decrease in reduction potential with pH is invariably observed in the pH range where deprotonation of the imidazolium nitrogen of histidine residue(s) occurs. No pH dependence is observed for the only protein lacking histidines. It appears that surface charges like the His imidazolium groups are capable of influencing the reduction potential despite the known quencing of the electrostatic interactions due to solvent effects.
INFLUENCE OF SURFACE-CHARGES ON REDOX PROPERTIES IN HIGH-POTENTIAL IRON-SULFUR PROTEINS / Luchinat, C; Capozzi, F; Borsari, Marco; Battistuzzi, Gianantonio; Sola, Marco. - In: BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS. - ISSN 0006-291X. - STAMPA. - 203:(1994), pp. 436-442. [10.1006/bbrc.1994.2201]
INFLUENCE OF SURFACE-CHARGES ON REDOX PROPERTIES IN HIGH-POTENTIAL IRON-SULFUR PROTEINS
BORSARI, Marco;BATTISTUZZI, Gianantonio;SOLA, Marco
1994
Abstract
The pH-dependence of the reduction potential determined through differential pulse voltammetry for the high potential iron sulfur proteins (HiPIP) from R. globiformis, C. vinosum, R. gelatinosus, E. vacuolata (I and II), E. halophila (I and II) is reported. A decrease in reduction potential with pH is invariably observed in the pH range where deprotonation of the imidazolium nitrogen of histidine residue(s) occurs. No pH dependence is observed for the only protein lacking histidines. It appears that surface charges like the His imidazolium groups are capable of influencing the reduction potential despite the known quencing of the electrostatic interactions due to solvent effects.
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