The effects of peroxidation on the erythrocytes of rats orally treated with L-propionyl carnitine for 15 day) (50 mg/kg/day/ were investigated. Peroxidation was produced by incubating the cells in the presence of the cytotoxic system: lactoperoxidase-hydrogen peroxide and iodide ions. Lysis of erythrocytes was evaluated by measuring the turbidity following the decrease in absorbance at 600 nm. The 50% of erythrocyte lysis of untreated animals was observed after 16 min and in about 30 min all the cells were lysed. With L-propionyl carnitine-treated rat erythrocytes the time at which 50% of lysis was observed increased to 23 min. L-propionyl carnitine also exerted its protective effect in vitro when incubated with untreated rat erythrocytes or human erythrocytes in the presence of the cytolytic system. The presence of L-propionyl carnitine in the incubation mixture markedly decreased the malonaldehyde formation. The protection was concentration-dependent. To establish if L-propionyl carnitine protects from oxygen reactive species or is able to stabilize the damaged membranes, a latent damage was produced by incubating the erythrocytes with the cytolytic system for a few minutes. The cells were then removed and suspended in buffered saline in the absence or in the presence of different L-propionyl carnitine concentrations L-propionyl carnitine decreased the velocity of lysis of damaged erythrocytes. These data suggest that L-propionyl carnitine protects erythrocytes from oxygen reactive species and also stabilizes the damaged membrane probably by specific binding with protein and/or phospholipid domains. Low density lipoproteins (LDLs) from human blood were peroxidized by exposure to Cu2+ ions in the presence of various L-propionyl carnitine concentrations. The formation of malonaldehyde decreased in the presence of L-propionyl carnitine, These findings provide evidence that L-propionyl carnitine protects cell membrane and circulating lipoproteins from peroxidation and stabilizes the cell membrane damaged by oxygen reactive species.

L-PROPIONYL CARNITINE PROTECTS ERYTHROCYTES AND LOW-DENSITY LIPOPROTEINS AGAINST PEROXIDATION / Bertelli, A; Conte, Angela; Ronca, G.. - In: DRUGS UNDER EXPERIMENTAL AND CLINICAL RESEARCH. - ISSN 0378-6501. - STAMPA. - 20:(1994), pp. 191-197.

L-PROPIONYL CARNITINE PROTECTS ERYTHROCYTES AND LOW-DENSITY LIPOPROTEINS AGAINST PEROXIDATION

CONTE, Angela;
1994

Abstract

The effects of peroxidation on the erythrocytes of rats orally treated with L-propionyl carnitine for 15 day) (50 mg/kg/day/ were investigated. Peroxidation was produced by incubating the cells in the presence of the cytotoxic system: lactoperoxidase-hydrogen peroxide and iodide ions. Lysis of erythrocytes was evaluated by measuring the turbidity following the decrease in absorbance at 600 nm. The 50% of erythrocyte lysis of untreated animals was observed after 16 min and in about 30 min all the cells were lysed. With L-propionyl carnitine-treated rat erythrocytes the time at which 50% of lysis was observed increased to 23 min. L-propionyl carnitine also exerted its protective effect in vitro when incubated with untreated rat erythrocytes or human erythrocytes in the presence of the cytolytic system. The presence of L-propionyl carnitine in the incubation mixture markedly decreased the malonaldehyde formation. The protection was concentration-dependent. To establish if L-propionyl carnitine protects from oxygen reactive species or is able to stabilize the damaged membranes, a latent damage was produced by incubating the erythrocytes with the cytolytic system for a few minutes. The cells were then removed and suspended in buffered saline in the absence or in the presence of different L-propionyl carnitine concentrations L-propionyl carnitine decreased the velocity of lysis of damaged erythrocytes. These data suggest that L-propionyl carnitine protects erythrocytes from oxygen reactive species and also stabilizes the damaged membrane probably by specific binding with protein and/or phospholipid domains. Low density lipoproteins (LDLs) from human blood were peroxidized by exposure to Cu2+ ions in the presence of various L-propionyl carnitine concentrations. The formation of malonaldehyde decreased in the presence of L-propionyl carnitine, These findings provide evidence that L-propionyl carnitine protects cell membrane and circulating lipoproteins from peroxidation and stabilizes the cell membrane damaged by oxygen reactive species.
1994
20
191
197
L-PROPIONYL CARNITINE PROTECTS ERYTHROCYTES AND LOW-DENSITY LIPOPROTEINS AGAINST PEROXIDATION / Bertelli, A; Conte, Angela; Ronca, G.. - In: DRUGS UNDER EXPERIMENTAL AND CLINICAL RESEARCH. - ISSN 0378-6501. - STAMPA. - 20:(1994), pp. 191-197.
Bertelli, A; Conte, Angela; Ronca, G.
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11380/10395
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