Multiple sclerosis (MS) is an autoimmune demyelinating disorder of the central nervous system (CNS) characterized by loss of myelin accompanied by infiltration of T-lymphocytes and monocytes. Although it has been shown that these infiltrates are important for the progression of MS, the role of microglia, the resident macrophages of the CNS, remains ambiguous. Therefore, we have compared the phenotypes of microglia and macrophages in a mouse model for MS, experimental autoimmune encephalomyelitis (EAE). In order to properly discriminate between these two cell types, microglia were defined as CD11bpos CD45int Ly-6Cneg, and infiltrated macrophages as CD11bpos CD45high Ly-6Cpos. During clinical EAE, microglia displayed a weakly immune-activated phenotype, based on the expression of MHCII, co-stimulatory molecules (CD80, CD86, and CD40) and proinflammatory genes [interleukin-1b (IL-1b) and tumour necrosis factor-a (TNF-a)]. In contrast, CD11bpos CD45high Ly-6Cpos infiltrated macrophages were strongly activated and could be divided into two populations Ly-6Cint and Ly-6Chigh, respectively. Ly-6Chigh macrophages contained less myelin than Ly-6Cint macrophages and expression levels of the proinflammatory cytokines IL-1b and TNF-a were higher in Ly-6Cint macrophages. Together, our data show that during clinical EAE, microglia are only weakly activated whereas infiltrated macrophages are highly immune reactive.

In acute experimental autoimmune encephalomyelitis, infiltrating macrophages are immune activated, whereas microglia remain immune suppressed / Vainchtein, Id; Vinet, Jonathan; Brouwer, N; Brendecke, S; Biagini, Giuseppe; Biber, K; Boddeke, Hw; Eggen, Bj. - In: GLIA. - ISSN 0894-1491. - STAMPA. - 62:(2014), pp. 1724-1735. [10.1002/glia.22711]

In acute experimental autoimmune encephalomyelitis, infiltrating macrophages are immune activated, whereas microglia remain immune suppressed.

VINET, JONATHAN;BIAGINI, Giuseppe;
2014

Abstract

Multiple sclerosis (MS) is an autoimmune demyelinating disorder of the central nervous system (CNS) characterized by loss of myelin accompanied by infiltration of T-lymphocytes and monocytes. Although it has been shown that these infiltrates are important for the progression of MS, the role of microglia, the resident macrophages of the CNS, remains ambiguous. Therefore, we have compared the phenotypes of microglia and macrophages in a mouse model for MS, experimental autoimmune encephalomyelitis (EAE). In order to properly discriminate between these two cell types, microglia were defined as CD11bpos CD45int Ly-6Cneg, and infiltrated macrophages as CD11bpos CD45high Ly-6Cpos. During clinical EAE, microglia displayed a weakly immune-activated phenotype, based on the expression of MHCII, co-stimulatory molecules (CD80, CD86, and CD40) and proinflammatory genes [interleukin-1b (IL-1b) and tumour necrosis factor-a (TNF-a)]. In contrast, CD11bpos CD45high Ly-6Cpos infiltrated macrophages were strongly activated and could be divided into two populations Ly-6Cint and Ly-6Chigh, respectively. Ly-6Chigh macrophages contained less myelin than Ly-6Cint macrophages and expression levels of the proinflammatory cytokines IL-1b and TNF-a were higher in Ly-6Cint macrophages. Together, our data show that during clinical EAE, microglia are only weakly activated whereas infiltrated macrophages are highly immune reactive.
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1724
1735
In acute experimental autoimmune encephalomyelitis, infiltrating macrophages are immune activated, whereas microglia remain immune suppressed / Vainchtein, Id; Vinet, Jonathan; Brouwer, N; Brendecke, S; Biagini, Giuseppe; Biber, K; Boddeke, Hw; Eggen, Bj. - In: GLIA. - ISSN 0894-1491. - STAMPA. - 62:(2014), pp. 1724-1735. [10.1002/glia.22711]
Vainchtein, Id; Vinet, Jonathan; Brouwer, N; Brendecke, S; Biagini, Giuseppe; Biber, K; Boddeke, Hw; Eggen, Bj
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Utilizza questo identificativo per citare o creare un link a questo documento: http://hdl.handle.net/11380/1022713
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