The effect of L-propionyl carnitine, the carnitine derivative utilized as a more effective drug for membrane protection, on Na-K ATPase activity of erythrocyte gh os ts of alcohol-dependent patients and blood donors has been investigated The effect of L-propionyl carnitine on leukocyte chemotaxis and cytochrome c reduction, a measure of superoxide ion production, was also studied. It has been in fact observed that alcohol is immunotoxic on both the non-specific and the specific immune response. In alcohol-dependent erythrocytes, a significant higher value of the H-1-NMR spin lattice relaxation time (T1) was observed as compared to blood-donor erythrocytes. The in-vitro addition of ethanol increases the T1 values of blood donor erythrocytes, whereas it is without effect on the T1 value of alcohol-dependent erythrocytes. The Na-K ATPase activity is higher in alcohol-dependent erythrocyte ghosts as compared to blood-donor ghosts. A non-significant increase of the Na-K ATPase activity of blood-donor ghosts was observed with the increasing of L-propionyl carnitine concentrations (from 0.2 to 5.0 mM), whereas the Na-K ATPase activity of alcohol-dependent ghosts decreases. From these combined effects the differences of Na-K ATPase activity progressively decrease with the increasing of L-propionyl carnitine concentration, and no significant differences are observed between the two groups at L-propionyl carnitine concentrations higher than 0.5 mM. The in-vitro addition of ethanol increases the enzyme activity to a greater extent in blood-donor ghosts as compared to alcohol-dependent ghosts. This in-vitro activation by ethanol is decreased by the addition of L-propionyl carnitine. The chemotaxis induced by N-formyl-methionyl-leucylphenylalanine and the superoxide anion production stimulated by zymosan is significantly lower in alcohol-dependent neutrophils. L-Propionyl carnitine increases, in a dose-dependent way, both chemotaxis and superoxide anion production of alcohol-dependent neutrophils, and no significant difference was observed between the two groups at 5 mM L-propionyl carnitine. These experimental results suggest that L-propionyl carnitine administration may be useful for reducing some acute and chronic damages due to alcohol ingestion. The protective and modulatory actions of L-propionyl carnitine may be even more evident in cells and tissues different from those investigated in this study and in which ethanol determines several biochemical damages.

EFFECT OF L-PROPIONYL CARNITINE ON SOME PROPERTIES OF ERYTHROCYTES AND LEUKOCYTES OF ALCOHOL ABUSERS / Conte, Angela; Bianchi, I; Guazzelli, M; Taponeco, G; Bertelli, A; Ronca, G.. - In: INTERNATIONAL JOURNAL OF TISSUE REACTIONS. - ISSN 0250-0868. - STAMPA. - 17:(1995), pp. 21-31.

EFFECT OF L-PROPIONYL CARNITINE ON SOME PROPERTIES OF ERYTHROCYTES AND LEUKOCYTES OF ALCOHOL ABUSERS

CONTE, Angela;
1995-01-01

Abstract

The effect of L-propionyl carnitine, the carnitine derivative utilized as a more effective drug for membrane protection, on Na-K ATPase activity of erythrocyte gh os ts of alcohol-dependent patients and blood donors has been investigated The effect of L-propionyl carnitine on leukocyte chemotaxis and cytochrome c reduction, a measure of superoxide ion production, was also studied. It has been in fact observed that alcohol is immunotoxic on both the non-specific and the specific immune response. In alcohol-dependent erythrocytes, a significant higher value of the H-1-NMR spin lattice relaxation time (T1) was observed as compared to blood-donor erythrocytes. The in-vitro addition of ethanol increases the T1 values of blood donor erythrocytes, whereas it is without effect on the T1 value of alcohol-dependent erythrocytes. The Na-K ATPase activity is higher in alcohol-dependent erythrocyte ghosts as compared to blood-donor ghosts. A non-significant increase of the Na-K ATPase activity of blood-donor ghosts was observed with the increasing of L-propionyl carnitine concentrations (from 0.2 to 5.0 mM), whereas the Na-K ATPase activity of alcohol-dependent ghosts decreases. From these combined effects the differences of Na-K ATPase activity progressively decrease with the increasing of L-propionyl carnitine concentration, and no significant differences are observed between the two groups at L-propionyl carnitine concentrations higher than 0.5 mM. The in-vitro addition of ethanol increases the enzyme activity to a greater extent in blood-donor ghosts as compared to alcohol-dependent ghosts. This in-vitro activation by ethanol is decreased by the addition of L-propionyl carnitine. The chemotaxis induced by N-formyl-methionyl-leucylphenylalanine and the superoxide anion production stimulated by zymosan is significantly lower in alcohol-dependent neutrophils. L-Propionyl carnitine increases, in a dose-dependent way, both chemotaxis and superoxide anion production of alcohol-dependent neutrophils, and no significant difference was observed between the two groups at 5 mM L-propionyl carnitine. These experimental results suggest that L-propionyl carnitine administration may be useful for reducing some acute and chronic damages due to alcohol ingestion. The protective and modulatory actions of L-propionyl carnitine may be even more evident in cells and tissues different from those investigated in this study and in which ethanol determines several biochemical damages.
17
21
31
EFFECT OF L-PROPIONYL CARNITINE ON SOME PROPERTIES OF ERYTHROCYTES AND LEUKOCYTES OF ALCOHOL ABUSERS / Conte, Angela; Bianchi, I; Guazzelli, M; Taponeco, G; Bertelli, A; Ronca, G.. - In: INTERNATIONAL JOURNAL OF TISSUE REACTIONS. - ISSN 0250-0868. - STAMPA. - 17:(1995), pp. 21-31.
Conte, Angela; Bianchi, I; Guazzelli, M; Taponeco, G; Bertelli, A; Ronca, G.
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11380/10039
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