Glycosaminoglycans (GAGs) from breast cyst fluid (BCF) of gross cysts, subdivided into apocrine and flattened, directly collected from 27 gross-cystic-breast-disease (GCBD)-affected women were analysed. Heparan sulfate, not further investigated, and chondroitin sulfate were identified. This last polysaccharide, in a content of 25-27 µg ml(-1) BCF and having a high molecular mass (~20 000-22 000), was found rich in glucuronic acid (~96%-98%) and mainly sulfated in position 4 of the N-acetyl-galactosamine (~60%-64%). Moreover, the presence of ~19%-24% of uncommon 4,6-O-disulfated disaccharides CS-E inside the polysaccharide chains with a high charge density of ~1.15-1.20 was determined. No substantial differences between apocrine and flattened cysts were observed. The current study describes the first effort to examine the yield and distribution of complex macromolecules like GAGs in BCF, and the understanding of their structure may help explain some functions associated with physiological and pathological conditions.

Characterization of Oversulfated Chondroitin Sulfate Rich in 4,6-O-disulfated Disaccharides in Breast Cyst Fluids Collected from Human Breast Gross Cysts / Mannello, F; Maccari, Francesca; Ligi, D; Canale, M; Galeotti, Fabio; Volpi, Nicola. - In: CELL BIOCHEMISTRY AND FUNCTION. - ISSN 0263-6484. - STAMPA. - 32(4)(2014), pp. 344-350. [10.1002/cbf.3022]

Characterization of Oversulfated Chondroitin Sulfate Rich in 4,6-O-disulfated Disaccharides in Breast Cyst Fluids Collected from Human Breast Gross Cysts.

MACCARI, Francesca;GALEOTTI, FABIO;VOLPI, Nicola
2014

Abstract

Glycosaminoglycans (GAGs) from breast cyst fluid (BCF) of gross cysts, subdivided into apocrine and flattened, directly collected from 27 gross-cystic-breast-disease (GCBD)-affected women were analysed. Heparan sulfate, not further investigated, and chondroitin sulfate were identified. This last polysaccharide, in a content of 25-27 µg ml(-1) BCF and having a high molecular mass (~20 000-22 000), was found rich in glucuronic acid (~96%-98%) and mainly sulfated in position 4 of the N-acetyl-galactosamine (~60%-64%). Moreover, the presence of ~19%-24% of uncommon 4,6-O-disulfated disaccharides CS-E inside the polysaccharide chains with a high charge density of ~1.15-1.20 was determined. No substantial differences between apocrine and flattened cysts were observed. The current study describes the first effort to examine the yield and distribution of complex macromolecules like GAGs in BCF, and the understanding of their structure may help explain some functions associated with physiological and pathological conditions.
32(4)
344
350
Characterization of Oversulfated Chondroitin Sulfate Rich in 4,6-O-disulfated Disaccharides in Breast Cyst Fluids Collected from Human Breast Gross Cysts / Mannello, F; Maccari, Francesca; Ligi, D; Canale, M; Galeotti, Fabio; Volpi, Nicola. - In: CELL BIOCHEMISTRY AND FUNCTION. - ISSN 0263-6484. - STAMPA. - 32(4)(2014), pp. 344-350. [10.1002/cbf.3022]
Mannello, F; Maccari, Francesca; Ligi, D; Canale, M; Galeotti, Fabio; Volpi, Nicola
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Utilizza questo identificativo per citare o creare un link a questo documento: http://hdl.handle.net/11380/988907
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