Expression of carbonic anhydrase, cystic fibrosis transmembrane regulator (CFTR) and V-H+-ATPase in the lancelet Branchiostoma lanceolatum (Pallas, 1774)

Sequencing of the amphioxus genome revealed that it contains a basic set of chordate genes involved in development and cell signalling. Despite the availability of genomic data, up till now no studies have been addressed on the comprehension of the amphioxus osmoregulation. Using primers designed on Branchiostoma floridae carbonic anhydrase (CA) II, cystic fibrosis transmembrane regulator (CFTR) and V-H+-ATPase, a 100bp long region, containing the protein region recognized by the respective antibodies, has been amplified and sequenced in B. lanceolatum indicating the presence of hortologous V-ATPase, CFTR and carbonic anhydrase II genes in Branchiostoma lanceolatum. Immunohistochemical results showed that all three transporting proteins are expressed in almost 90% of epithelial cells of skin in B. lanceolatum adults with a different degree of positivity in different regions of body wall and with a different localization into the cells. The comparison of results between young and adult lancelets showed that the distribution of these transporters is quite different. Indeed, in the young specimens the expression pattern of all tested molecules appears concentrated at the gut level, whereas in adult the gut loses its key role that is mostly supported by skin.