Bis-(2-ethylhexyl) phthalate (DEHP) is a widely used industrial additive for increasing plastic !exibility. Its metabolites are known to exert toxic effects on reproduction and development of mammals. The aim of this study was to evaluate the effects of environmentally relevant concentrations of DEHP (0.2 and 20 mg/L) on the reproductive biology of adult male zebra"sh (Danio rerio). The effects of DEHP and 17b-ethynylestradiol (a positive control) were determined after one or three weeks of exposure by TUNEL assay, histomorphometric analysis and evaluation of reproductive performance. DEHP impaired reproduction in zebra"sh by inducing a mitotic arrest during spermatogenesis, increasing DNA fragmentation in sperm cells and markedly reducing embryo production (up to 90%). In conclusion, relatively shortterm exposure to environmentally relevant concentrations of DEHP is able to alter spermatogenesis and affect reproduction in zebra"sh.
Bis (2-ethylexhyl) phthalate impairs spermiogenesis in zebrafish (Danio rerio) / Corradetti, B; Stronati, A; Tosti, L; Manicardi, Gian Carlo; Carnevali, O; Bizzaro, D.. - In: REPRODUCTIVE BIOLOGY. - ISSN 1642-431X. - STAMPA. - 13:3(2013), pp. 195-202. [10.1016/j.repbio.2013.07.003]
Bis (2-ethylexhyl) phthalate impairs spermiogenesis in zebrafish (Danio rerio)
MANICARDI, Gian Carlo;
2013
Abstract
Bis-(2-ethylhexyl) phthalate (DEHP) is a widely used industrial additive for increasing plastic !exibility. Its metabolites are known to exert toxic effects on reproduction and development of mammals. The aim of this study was to evaluate the effects of environmentally relevant concentrations of DEHP (0.2 and 20 mg/L) on the reproductive biology of adult male zebra"sh (Danio rerio). The effects of DEHP and 17b-ethynylestradiol (a positive control) were determined after one or three weeks of exposure by TUNEL assay, histomorphometric analysis and evaluation of reproductive performance. DEHP impaired reproduction in zebra"sh by inducing a mitotic arrest during spermatogenesis, increasing DNA fragmentation in sperm cells and markedly reducing embryo production (up to 90%). In conclusion, relatively shortterm exposure to environmentally relevant concentrations of DEHP is able to alter spermatogenesis and affect reproduction in zebra"sh.
Pubblicazioni consigliate
I metadati presenti in IRIS UNIMORE sono rilasciati con licenza Creative Commons CC0 1.0 Universal, mentre i file delle pubblicazioni sono rilasciati con licenza Attribuzione 4.0 Internazionale (CC BY 4.0), salvo diversa indicazione.
In caso di violazione di copyright, contattare Supporto Iris
