This study is an investigation of the effect of biocompatible glutathione-covered gold nanoparticles (AuSG_7) with an average size of 3 nm on the amyloid fibrils of hen egg-white lysozyme. The anti-amyloid activity of AuSG_7 nanoparticles on this protein was monitored with thioflavin T assay, atomic force microscopy and transmission electron microscopy. The study found that AuSG_7 nanoparticles in vitro depolymerize the amyloid aggregates and inhibit lysozyme aggregate formation. The ability to inhibit amyloid formation and promote amyloid disassembly has concentration-dependent characteristics: the concentration of nanoparticles at which inhibition is half maximal (IC50) was found to be 6.19 mu g/mL, and the concentration at which depolymerization is half maximal (DC50) was found to be 826 mu g/ml. (C) 2012 Elsevier B.V. All rights reserved.

Anti-amyloidogenic activity of glutathione-covered gold nanoparticles / A., Antosova; Z., Gazova; D., Fedunova; E., Valusova; E., Bystrenova; F., Valle; Z., Daxnerova; Biscarini, Fabio; M., Antalik. - In: MATERIALS SCIENCE AND ENGINEERING. C, BIOMIMETIC MATERIALS, SENSORS AND SYSTEMS. - ISSN 0928-4931. - ELETTRONICO. - 32:8(2012), pp. 2529-2535. [10.1016/j.msec.2012.07.036]

Anti-amyloidogenic activity of glutathione-covered gold nanoparticles

BISCARINI, FABIO;
2012

Abstract

This study is an investigation of the effect of biocompatible glutathione-covered gold nanoparticles (AuSG_7) with an average size of 3 nm on the amyloid fibrils of hen egg-white lysozyme. The anti-amyloid activity of AuSG_7 nanoparticles on this protein was monitored with thioflavin T assay, atomic force microscopy and transmission electron microscopy. The study found that AuSG_7 nanoparticles in vitro depolymerize the amyloid aggregates and inhibit lysozyme aggregate formation. The ability to inhibit amyloid formation and promote amyloid disassembly has concentration-dependent characteristics: the concentration of nanoparticles at which inhibition is half maximal (IC50) was found to be 6.19 mu g/mL, and the concentration at which depolymerization is half maximal (DC50) was found to be 826 mu g/ml. (C) 2012 Elsevier B.V. All rights reserved.
2012
32
8
2529
2535
Anti-amyloidogenic activity of glutathione-covered gold nanoparticles / A., Antosova; Z., Gazova; D., Fedunova; E., Valusova; E., Bystrenova; F., Valle; Z., Daxnerova; Biscarini, Fabio; M., Antalik. - In: MATERIALS SCIENCE AND ENGINEERING. C, BIOMIMETIC MATERIALS, SENSORS AND SYSTEMS. - ISSN 0928-4931. - ELETTRONICO. - 32:8(2012), pp. 2529-2535. [10.1016/j.msec.2012.07.036]
A., Antosova; Z., Gazova; D., Fedunova; E., Valusova; E., Bystrenova; F., Valle; Z., Daxnerova; Biscarini, Fabio; M., Antalik
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11380/966494
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