Centenarians, particularly healthy centenarians, constitute the example of successful aging and the study of their immune status can help to define the endpoint of the changes occurring throughout life. We characterized T cell clones (TCC) of two healthy centenarians, studying their phenotypes and production of representative Th1 and Th2 cytokines (IFN-gamma and IL-4) and compared them with TCC obtained by three young normal subjects; in all 180 TCC were analyzed, In young donors, 35 TCC were CD4(+), 56 CD8(+) and 2 were alpha beta(+)CD4(-)CD8(-) (double negative). In centenarians, we obtained 46 CD4(+) TCC, 38 CD8(+), 2 CD4(+)CD8(+) (double positive) and 1 gamma delta(+) double negative. Of the young subjects' TCC, 71% produced IFN-gamma but no IL-4 (Th1 pattern) and this prevalence decreased to 39% in TCC from the centenarians. The number of clones showing the opposite Th2 pattern was similar in young and aged donors (3 out of 93 TCC and 2 out of 87 TCC, respectively). The intermediate profile of TCC producing both IL-4 and IFN-gamma (Th0) was found in 25.8% of clones from young people, but it almost doubled to 58.6% in centenarians. The analysis shows that the Th profiles of CD8(+) TCC is nearly superimposable in the two groups, whereas a major shift from a Th1 to a Th0 pattern is presented by CD4(+) TCC. The balance provided by a majority of CD4(+) TCC showing a Th0 pattern may ensure both humoral and cell-mediated defences. In CD8(+) TCC, however, a Th1 pattern still is present, possibly for efficient generation of cytotoxic responses. These findings should be extended by studying other centenarians and elderly subjects.
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|Anno di pubblicazione:||1996|
|Titolo:||A shift to Th0 cytokine production by CD4(+) cells in human longevity: Studies on two healthy centenarians|
|Autori:||Paganelli R; Scala E; Rosso R; Cossarizza A; Bertollo L; Barbieri D; Fabrizi A; Lusi EA; Fagiolo U; Franceschi C|
|Autori interni:||COSSARIZZA, Andrea|
|Rivista:||EUROPEAN JOURNAL OF IMMUNOLOGY|
|Appare nelle tipologie:||Articolo su rivista|
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