Cell therapy is an emerging therapeutic strategy aimed at replacing or repairing severely damaged tissues with cultured cells. Speci fi cally, ocular burns cause depletion of limbal stem cells, which leads to corneal opaci fi cation and visual loss. Corneal stem cells are segregated in the basal layer of the limbus, which is the transitional zone of the epithelium located between the cornea and the bulbar conjunctiva. Autologous cultured limbal epithelial cells can restore damaged corneas. We sought to establish a culture system that allows preservation of limbal stem cells and preparation of manageable epithelial sheets. We outline some quality criteria, which assure the clinical performance of keratinocyte culture: evaluation of the number of holoclones within a cultured epithelial graft, proportion of aborting colonies, and percentage of cells expressing high levels of D Np63 a .

Methods for characterization/manipulation of human corneal stem cells and their applications in regenerative medicine / Corradini, Francesca; Venturi, Beatrice; Pellegrini, Graziella; DE LUCA, Michele. - STAMPA. - 916:(2012), pp. 357-372. [10.1007/978-1-61779-980-8_26]

Methods for characterization/manipulation of human corneal stem cells and their applications in regenerative medicine.

CORRADINI, Francesca;VENTURI, BEATRICE;PELLEGRINI, Graziella;DE LUCA, Michele
2012

Abstract

Cell therapy is an emerging therapeutic strategy aimed at replacing or repairing severely damaged tissues with cultured cells. Speci fi cally, ocular burns cause depletion of limbal stem cells, which leads to corneal opaci fi cation and visual loss. Corneal stem cells are segregated in the basal layer of the limbus, which is the transitional zone of the epithelium located between the cornea and the bulbar conjunctiva. Autologous cultured limbal epithelial cells can restore damaged corneas. We sought to establish a culture system that allows preservation of limbal stem cells and preparation of manageable epithelial sheets. We outline some quality criteria, which assure the clinical performance of keratinocyte culture: evaluation of the number of holoclones within a cultured epithelial graft, proportion of aborting colonies, and percentage of cells expressing high levels of D Np63 a .
2012
Methods in Molecular Biology
Methods for characterization/manipulation of human corneal stem cells and their applications in regenerative medicine / Corradini, Francesca; Venturi, Beatrice; Pellegrini, Graziella; DE LUCA, Michele. - STAMPA. - 916:(2012), pp. 357-372. [10.1007/978-1-61779-980-8_26]
Corradini, Francesca; Venturi, Beatrice; Pellegrini, Graziella; DE LUCA, Michele
File in questo prodotto:
Non ci sono file associati a questo prodotto.
Pubblicazioni consigliate

Licenza Creative Commons
I metadati presenti in IRIS UNIMORE sono rilasciati con licenza Creative Commons CC0 1.0 Universal, mentre i file delle pubblicazioni sono rilasciati con licenza Attribuzione 4.0 Internazionale (CC BY 4.0), salvo diversa indicazione.
In caso di violazione di copyright, contattare Supporto Iris

Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11380/864295
Citazioni
  • ???jsp.display-item.citation.pmc??? 5
  • Scopus 16
  • ???jsp.display-item.citation.isi??? ND
social impact