The present paper describes the possibility of determination of mitochondrial membrane potential (Delta omega) in isolated hepatocytes making use of a Delta psi-sensitive dye, i.e., the lipophilic cationic probe 5,5'6,6'-tetrachloro-1,1'3,3 '-tetraethylbenzimidazolcarbocyanine iodide (JC-1) and of cytofluorimetry. The validity of the method was proved by treating hepatocytes with FCCP (decrease of Delta psi) and subsequent addition of 6-ketocholestanol (increase of Delta psi). The results indicate that the proposed method may be used in laboratory practice.
Use of flow cytometry as a tool to study mitochondrial membrane potential in isolated, living hepatocytes / Salvioli, S; Maseroli, R; Pazienza, Tl; Bobyleva, V; Cossarizza, Andrea. - In: BIOCHEMISTRY. - ISSN 0006-2979. - ELETTRONICO. - 63:(1998), pp. 235-238.
Use of flow cytometry as a tool to study mitochondrial membrane potential in isolated, living hepatocytes
COSSARIZZA, Andrea
1998
Abstract
The present paper describes the possibility of determination of mitochondrial membrane potential (Delta omega) in isolated hepatocytes making use of a Delta psi-sensitive dye, i.e., the lipophilic cationic probe 5,5'6,6'-tetrachloro-1,1'3,3 '-tetraethylbenzimidazolcarbocyanine iodide (JC-1) and of cytofluorimetry. The validity of the method was proved by treating hepatocytes with FCCP (decrease of Delta psi) and subsequent addition of 6-ketocholestanol (increase of Delta psi). The results indicate that the proposed method may be used in laboratory practice.
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