The identification of f)-endorphin (f)-EP) as part of the larger precurs~_ f)-lipotropin (f)-LPH) in pituitary cells and the importance of f)-E P in behaviour and endogenous analgesia dictate the need for a method of measur- ing the plasma or li.quour concentrations of them in physiological and path- ological conditions. The present study concerns the deve:.opment of RIA methods to measure f)-EP ~:Id f)-LPH after extraction and pu r i f i c a t i.o n . Synt:" etic f)-EP (Peninsular Inc., San Francisco) and h f)-LPH (Dr.C.H.Li, San Fra~ cisco) and h f)-EP antiserum (Dr.C.Pert NIH, Bethesda) were used. 8nth peptides were labelled with Il~5 using the chloramine T method, an then purified by gel filtration in a Sephadex G-25 culumn. The specific activity of f)-EP-I125 was lSOuC/ug and that of f)-LPH-I125 was 242uC/ug. The specificity of the antiserum using both labelled peptides was chec~ with the following substances: leu-enkephalin, Ret-enkephalin, substance P GIF, 1-39 ACTH, f)-MSH, 61-77 f)-LPH, 79-91 f)-LPH, f)-E P and f)-LPH. No cross reaction was found with leu and met-enkephalin, substance P, GIF and 1-39 ACTH, while in the f)-EP and f)-LPH RIAs, f)-LPH and f)-EP showe d a cross r e ac t-:
SIMULTANEOUS RIA OF HUMAN BETA-ENDORPHIN AND BETA-LIPOTROPIN IN PLASMA EXTRACTS / Facchinetti, Fabio; Genazzani, Ar. - In: ACTA ENDOCRINOLOGICA. - ISSN 0001-5598. - STAMPA. - 225:(1979), pp. 378-378. (Intervento presentato al convegno XIIth acta endocrinologica congress tenutosi a munich nel 26-30 june 1979).
SIMULTANEOUS RIA OF HUMAN BETA-ENDORPHIN AND BETA-LIPOTROPIN IN PLASMA EXTRACTS
FACCHINETTI, Fabio;
1979
Abstract
The identification of f)-endorphin (f)-EP) as part of the larger precurs~_ f)-lipotropin (f)-LPH) in pituitary cells and the importance of f)-E P in behaviour and endogenous analgesia dictate the need for a method of measur- ing the plasma or li.quour concentrations of them in physiological and path- ological conditions. The present study concerns the deve:.opment of RIA methods to measure f)-EP ~:Id f)-LPH after extraction and pu r i f i c a t i.o n . Synt:" etic f)-EP (Peninsular Inc., San Francisco) and h f)-LPH (Dr.C.H.Li, San Fra~ cisco) and h f)-EP antiserum (Dr.C.Pert NIH, Bethesda) were used. 8nth peptides were labelled with Il~5 using the chloramine T method, an then purified by gel filtration in a Sephadex G-25 culumn. The specific activity of f)-EP-I125 was lSOuC/ug and that of f)-LPH-I125 was 242uC/ug. The specificity of the antiserum using both labelled peptides was chec~ with the following substances: leu-enkephalin, Ret-enkephalin, substance P GIF, 1-39 ACTH, f)-MSH, 61-77 f)-LPH, 79-91 f)-LPH, f)-E P and f)-LPH. No cross reaction was found with leu and met-enkephalin, substance P, GIF and 1-39 ACTH, while in the f)-EP and f)-LPH RIAs, f)-LPH and f)-EP showe d a cross r e ac t-:
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