Background. Wide spectrum serological screening of women prior to or during pregnancy may greatly help in preventing vertically transmitted infections (VTI) and their severe consequences to the foetus/newborn. Protein microarrays, made up by spotting many antigens onto a restricted area of a microscope slide, allow to detect, in one shot, specific antibodies against a wide range of antigenic specificities. Objectives. To detect, in paired serum and follicular fluid (FF) samples, antigen-specific antibodies against vertically transmitted pathogens by protein microarrays; to investigate the potential relation between antibody profiles and pregnancy outcome. Methods. Serum and FF paired samples were collected from 102 women undergoing in vitro fertilization (IVF). Antigens, human antibodies and controls were spotted in an orderly manner by high speed robotics. Microarrays were processed with serum or FF and the occurred immunocomplexes were revealed by fluorescently-labelled secondary antibodies. The fluorescent signals were read by a laser scanner and quantified by a dedicated software. Conclusions. Antigen-specific antibodies can be effectively detected in serum and FF by microarray. The presence or absence of certain antigen-specific antibodies is significantly related to clinical parameters such as the number of inseminated good quality oocytes or the number of successful embryo transfers. These results encourage protein microarrays employment for wide spectrum investigations in diagnosing VTI; in particular, the use of biological matrices other than serum may help addressing yet unravelled questions.
|Titolo:||DETECTION OF ANTIGEN-SPECIFIC ANTIBODIES IN SERUM AND FOLLICULAR FLUIDS BY PROTEIN MICROARRAYS.|
|Autori:||Ardizzoni, Andrea; Baschieri, Maria Cristina; Manca, Lidia; Capodanno, Francesco; La Sala, Giovanni Battista; Blasi, Elisabetta|
|Data di pubblicazione:||2011|
|Appare nelle tipologie:||Poster|
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