EnglishA human ornithine decarboxylase (ODC) gene probe has been isolated from a Jurkat T-cell cDNA expression library, sequenced, and used to analyze ODC mRNA levels in untransformed human lymphocytes and fibroblasts stimulated to proliferate by various mitogens. The partial cDNA sequence is 86% homologous to the mouse ODC cDNA, and Northern blots indicate that the human and mouse mRNA species are similar in size. ODC mRNA is barely detectable in quiescent human T lymphocytes and undetectable in density-arrested W138 fibroblasts. Following stimulation of T-lymphocyte proliferation with phytohemagglutinin, the ODC mRNA level rises to a peak around mid G1 phase and decreases as the cells enter S phase. Serum stimulation of density-arrested fibroblasts results in an elevation of the ODC mRNA level which persists throughout the cell cycle. Epidermal growth factor (20 ng/ml) but not insulin (10 mg/ml) or dexamethasone (55 ng/ml) stimulates ODC expression in quiescent W138 fibroblasts. Southern blots suggest that human cells have a single copy of the ODC gene.
| Data di pubblicazione: | 1987 |
| Titolo: | Cell-cycle-dependent expression of human ornithine decarboxylase. |
| Autore/i: | Kaczmarek, L; Calabretta, Bruno; Ferrari, Sergio; de Riel, J. K. |
| Autore/i UNIMORE: | |
| Rivista: | |
| Volume: | 132 |
| Pagina iniziale: | 545 |
| Pagina finale: | 551 |
| Codice identificativo ISI: | WOS:A1987K286000017 |
| Codice identificativo Scopus: | 2-s2.0-0023492498 |
| Codice identificativo Pubmed: | 3308908 |
| Citazione: | Cell-cycle-dependent expression of human ornithine decarboxylase / Kaczmarek, L; Calabretta, Bruno; Ferrari, Sergio; de Riel, J. K.. - In: JOURNAL OF CELLULAR PHYSIOLOGY. - ISSN 0021-9541. - STAMPA. - 132(1987), pp. 545-551. |
| Tipologia | Articolo su rivista |
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