A human ornithine decarboxylase (ODC) gene probe has been isolated from a Jurkat T-cell cDNA expression library, sequenced, and used to analyze ODC mRNA levels in untransformed human lymphocytes and fibroblasts stimulated to proliferate by various mitogens. The partial cDNA sequence is 86% homologous to the mouse ODC cDNA, and Northern blots indicate that the human and mouse mRNA species are similar in size. ODC mRNA is barely detectable in quiescent human T lymphocytes and undetectable in density-arrested W138 fibroblasts. Following stimulation of T-lymphocyte proliferation with phytohemagglutinin, the ODC mRNA level rises to a peak around mid G1 phase and decreases as the cells enter S phase. Serum stimulation of density-arrested fibroblasts results in an elevation of the ODC mRNA level which persists throughout the cell cycle. Epidermal growth factor (20 ng/ml) but not insulin (10 mg/ml) or dexamethasone (55 ng/ml) stimulates ODC expression in quiescent W138 fibroblasts. Southern blots suggest that human cells have a single copy of the ODC gene.
Cell-cycle-dependent expression of human ornithine decarboxylase / Kaczmarek, L; Calabretta, Bruno; Ferrari, Sergio; de Riel, J. K.. - In: JOURNAL OF CELLULAR PHYSIOLOGY. - ISSN 0021-9541. - STAMPA. - 132(1987), pp. 545-551.
Data di pubblicazione: | 1987 | |
Titolo: | Cell-cycle-dependent expression of human ornithine decarboxylase. | |
Autore/i: | Kaczmarek, L; Calabretta, Bruno; Ferrari, Sergio; de Riel, J. K. | |
Autore/i UNIMORE: | ||
Rivista: | ||
Volume: | 132 | |
Pagina iniziale: | 545 | |
Pagina finale: | 551 | |
Codice identificativo ISI: | WOS:A1987K286000017 | |
Codice identificativo Scopus: | 2-s2.0-0023492498 | |
Codice identificativo Pubmed: | 3308908 | |
Citazione: | Cell-cycle-dependent expression of human ornithine decarboxylase / Kaczmarek, L; Calabretta, Bruno; Ferrari, Sergio; de Riel, J. K.. - In: JOURNAL OF CELLULAR PHYSIOLOGY. - ISSN 0021-9541. - STAMPA. - 132(1987), pp. 545-551. | |
Tipologia | Articolo su rivista |
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