We have investigated the expression of certain cell cycle-dependent genes in total RNA isolated from normal and neoplastic cells of human epithelial colon tissue. The genes studied had been previously identified as cell cycle dependent in rodent and human fibroblasts. Levels of expression of G1 genes were compared to the level of expression of the S-phase-specific gene H3 in normal and adjacent neoplastic epithelial cells of six different individuals. We have found that the increase in the expression of c-myc gene detected in colon tumor cells is accompanied by a parallel increase in the expression of two G1-specific genes (p2A9 and ornithine decarboxylase) and the S-phase-specific gene histone H3. An important conclusion that can be drawn from these findings is that the increased level of a cell cycle-specific RNA in a tumor may not indicate overexpression of that gene but simply reflect the increased fraction of cycling cells, unless the ratio of expression between G1 genes and G1-S-phase genes is altered.

Expression of c-myc and other cell cycle-dependent genes in human colon neoplasia / Calabretta, Bruno; Kaczmarek, L; Ming, Pm; Au, F; Ming, S. C.. - In: CANCER RESEARCH. - ISSN 0008-5472. - STAMPA. - 45:(1985), pp. 6000-6004.

Expression of c-myc and other cell cycle-dependent genes in human colon neoplasia

CALABRETTA, Bruno;
1985

Abstract

We have investigated the expression of certain cell cycle-dependent genes in total RNA isolated from normal and neoplastic cells of human epithelial colon tissue. The genes studied had been previously identified as cell cycle dependent in rodent and human fibroblasts. Levels of expression of G1 genes were compared to the level of expression of the S-phase-specific gene H3 in normal and adjacent neoplastic epithelial cells of six different individuals. We have found that the increase in the expression of c-myc gene detected in colon tumor cells is accompanied by a parallel increase in the expression of two G1-specific genes (p2A9 and ornithine decarboxylase) and the S-phase-specific gene histone H3. An important conclusion that can be drawn from these findings is that the increased level of a cell cycle-specific RNA in a tumor may not indicate overexpression of that gene but simply reflect the increased fraction of cycling cells, unless the ratio of expression between G1 genes and G1-S-phase genes is altered.
45
6000
6004
Expression of c-myc and other cell cycle-dependent genes in human colon neoplasia / Calabretta, Bruno; Kaczmarek, L; Ming, Pm; Au, F; Ming, S. C.. - In: CANCER RESEARCH. - ISSN 0008-5472. - STAMPA. - 45:(1985), pp. 6000-6004.
Calabretta, Bruno; Kaczmarek, L; Ming, Pm; Au, F; Ming, S. C.
File in questo prodotto:
Non ci sono file associati a questo prodotto.
Pubblicazioni consigliate

Caricamento pubblicazioni consigliate

Licenza Creative Commons
I metadati presenti in IRIS UNIMORE sono rilasciati con licenza Creative Commons CC0 1.0 Universal, mentre i file delle pubblicazioni sono rilasciati con licenza Attribuzione 4.0 Internazionale (CC BY 4.0), salvo diversa indicazione.
In caso di violazione di copyright, contattare Supporto Iris

Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11380/811295
Citazioni
  • ???jsp.display-item.citation.pmc??? 13
  • Scopus 47
  • ???jsp.display-item.citation.isi??? 65
social impact