(4S)-(þ)-Carvone was totally reduced after 120 h by ER activity expressed by lyophilized whole cells of K. exigua DBVPG 7133 to (1S,4S)-dihydrocarvone (yield¼60%) and (1R,4S)-dihydrocarvone (40%). About 15% of (1S,4S)-dihydrocarvone was further reduced to (1S,2S,4S)-dihydrocarveol (7%) and (1S,2R,4S)-dihydrocarveol (8%). In close analogy with previous literature, the use of lyophilized yeast whole cells was generally preferred to growing and resting ones. Besides, because yeast cells contain only catalytic amount of NAD(P)H and cofactor regeneration must take place by means of metabolism of an electron donor, the simultaneous use of lyophilized yeast whole cells and glucose (the last acting as auxiliary substrate for cofactor-recycling systems) showed a significant increase of efficiency in reducing (4S)-(þ)-carvone.

Asymmetric reduction of (4S)-(+)-carvone catalyzed by enoate reductases (ERs) expressed by non-conventional yeast (NCY) whole-cells / P., Buzzini; M., Goretti; C., Ponzoni; E., Caselli; E., Branda; Cramarossa, Maria Rita; B., Turchetti; Forti, Luca. - STAMPA. - (2012), pp. 104-108.

Asymmetric reduction of (4S)-(+)-carvone catalyzed by enoate reductases (ERs) expressed by non-conventional yeast (NCY) whole-cells

CRAMAROSSA, Maria Rita;FORTI, Luca
2012

Abstract

(4S)-(þ)-Carvone was totally reduced after 120 h by ER activity expressed by lyophilized whole cells of K. exigua DBVPG 7133 to (1S,4S)-dihydrocarvone (yield¼60%) and (1R,4S)-dihydrocarvone (40%). About 15% of (1S,4S)-dihydrocarvone was further reduced to (1S,2S,4S)-dihydrocarveol (7%) and (1S,2R,4S)-dihydrocarveol (8%). In close analogy with previous literature, the use of lyophilized yeast whole cells was generally preferred to growing and resting ones. Besides, because yeast cells contain only catalytic amount of NAD(P)H and cofactor regeneration must take place by means of metabolism of an electron donor, the simultaneous use of lyophilized yeast whole cells and glucose (the last acting as auxiliary substrate for cofactor-recycling systems) showed a significant increase of efficiency in reducing (4S)-(þ)-carvone.
Practical Methods for Biocatalysis and Biotransformations 2
9781119991397
John Wiley & Sons, Ltd
REGNO UNITO DI GRAN BRETAGNA
Asymmetric reduction of (4S)-(+)-carvone catalyzed by enoate reductases (ERs) expressed by non-conventional yeast (NCY) whole-cells / P., Buzzini; M., Goretti; C., Ponzoni; E., Caselli; E., Branda; Cramarossa, Maria Rita; B., Turchetti; Forti, Luca. - STAMPA. - (2012), pp. 104-108.
P., Buzzini; M., Goretti; C., Ponzoni; E., Caselli; E., Branda; Cramarossa, Maria Rita; B., Turchetti; Forti, Luca
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11380/811125
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