Tetanus neurotoxin (TT), a product of microbial origin, acts as a zinc endopeptidase on vesicle-associated membrane proteins (VAMP). We have demonstrated that TT displays inhibitory effects on secretory and accessory functions in the murine macrophage (M phi) cell line GG2EE, Nitric oxide (NO) secretion was decreased when interferon (IFN)-gamma-pretreated GG2EE M phi s were coincubated with a fungal costimulus (SMP200) and TT, When heat-inactivated TT was used this effect was not evident, The TT-mediated phenomenon was dose-dependent and specific since, under the same experimental conditions, it did not affect interleukin-6 or tumor necrosis factor-alpha secretion. Furthermore, IFN-gamma-induced major histocompatibility complex class II molecule expression and GG2EE accessory function, assessed by SMP200-stimulated lymphoproliferation, were also inhibited by TT. Such inhibition was incomplete, in line with our previous results showing that TT partially cleaves VAMP proteins in murine M phi.
Tetanus toxin impairs accessory and secretory functions in interferon-gamma-treated murine macrophages / Pitzurra, L; Adami, C; Sevilla, M; Polonelli, L; Bistoni, F; Blasi, Elisabetta. - In: CELLULAR IMMUNOLOGY. - ISSN 0008-8749. - STAMPA. - 191 (1):(1999), pp. 20-25.
Tetanus toxin impairs accessory and secretory functions in interferon-gamma-treated murine macrophages
BLASI, Elisabetta
1999
Abstract
Tetanus neurotoxin (TT), a product of microbial origin, acts as a zinc endopeptidase on vesicle-associated membrane proteins (VAMP). We have demonstrated that TT displays inhibitory effects on secretory and accessory functions in the murine macrophage (M phi) cell line GG2EE, Nitric oxide (NO) secretion was decreased when interferon (IFN)-gamma-pretreated GG2EE M phi s were coincubated with a fungal costimulus (SMP200) and TT, When heat-inactivated TT was used this effect was not evident, The TT-mediated phenomenon was dose-dependent and specific since, under the same experimental conditions, it did not affect interleukin-6 or tumor necrosis factor-alpha secretion. Furthermore, IFN-gamma-induced major histocompatibility complex class II molecule expression and GG2EE accessory function, assessed by SMP200-stimulated lymphoproliferation, were also inhibited by TT. Such inhibition was incomplete, in line with our previous results showing that TT partially cleaves VAMP proteins in murine M phi.
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