The RT-PCR analysis of RNA from progenitor and differentiated primary rat oligodendrocytes, and from the oligodendrocyte CG-4 cell line, shows the presence of the IL-1 beta mRNA, the type I IL-1 receptor and the IL-1 receptor accessory protein in these cells. In situ hybridization of a rat IL-1 beta probe to primary progenitor and differentiated rat oligodendrocytes results in a positive signal. The double hybridization of the IL-1 beta probe, together with an oligodendrocyte-specific differentiation marker, to sections of postnatal rat brain at different stages of differentiation is also positive. The double immune-labelling technique utilized indicates coincidence of the signals on the brain slices, The results show that IL-1 beta mRNA is constitutively expressed in rat brain oligodendrocytes from 1 day after birth onward. In agreement with this observation, CG-4 cells, primary progenitor and differentiated rat oligodendrocytes are positively stained by antibodies against IL-1 beta, Postnatal brain slices from 1 and 4 day old and adult rats, labelled with a double immunofluorescence technique, are also stained by antibodies against IL-1 beta. This signal coincides with that of antibodies against oligodendrocyte-specific surface markers. We conclude that IL-1 beta is constitutively expressed in rat brain progenitor and differentiated oligodendrocytes.
Constitutive expression of interleukin-1 beta (IL-1 beta) in rat oligodendrocytes / F., Blasi; Riccio, Massimo; A., Brogi; M., Strazza; M. L., Taddei; S., Romagnoli; A., Luddi; R., D'Angelo; S., Santi; E., Costantino Ceccarini; M., Melli. - In: BIOLOGICAL CHEMISTRY. - ISSN 1431-6730. - STAMPA. - 380:(1999), pp. 259-264. [10.1515/BC.1999.034]
Constitutive expression of interleukin-1 beta (IL-1 beta) in rat oligodendrocytes
RICCIO, Massimo;
1999
Abstract
The RT-PCR analysis of RNA from progenitor and differentiated primary rat oligodendrocytes, and from the oligodendrocyte CG-4 cell line, shows the presence of the IL-1 beta mRNA, the type I IL-1 receptor and the IL-1 receptor accessory protein in these cells. In situ hybridization of a rat IL-1 beta probe to primary progenitor and differentiated rat oligodendrocytes results in a positive signal. The double hybridization of the IL-1 beta probe, together with an oligodendrocyte-specific differentiation marker, to sections of postnatal rat brain at different stages of differentiation is also positive. The double immune-labelling technique utilized indicates coincidence of the signals on the brain slices, The results show that IL-1 beta mRNA is constitutively expressed in rat brain oligodendrocytes from 1 day after birth onward. In agreement with this observation, CG-4 cells, primary progenitor and differentiated rat oligodendrocytes are positively stained by antibodies against IL-1 beta, Postnatal brain slices from 1 and 4 day old and adult rats, labelled with a double immunofluorescence technique, are also stained by antibodies against IL-1 beta. This signal coincides with that of antibodies against oligodendrocyte-specific surface markers. We conclude that IL-1 beta is constitutively expressed in rat brain progenitor and differentiated oligodendrocytes.
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