The intranuclear distribution of HMGI/Y proteins was analyzed by immunofluorescent staining in several cell lines using a polyclonal antibody that stained a fibrogranular network. In actively growing 3T3 fibroblasts, HMGI/Y proteins were mainly localized to heterochromatin masses, whereas in quiescent cells they were more diffusely distributed. Double labeling experiments showed a co-localization of HMGI/Y with DNA topoisomerase II alpha. These results are in agreement with previously published biochemical data and indicate a possible involvement of HMGI/Y proteins in several nuclear functions, including chromatin organization and gene expression.
Intranuclear distribution of HMGI/Y proteins: An immunocytochemical study / A. M., Martelli; Riccio, Massimo; R., Bareggi; G., Manfioletti; G., Tabellini; G., Baldini; P., Narducci; V., Giancotti. - In: JOURNAL OF HISTOCHEMISTRY & CYTOCHEMISTRY. - ISSN 0022-1554. - STAMPA. - 46:(1998), pp. 863-864.
Intranuclear distribution of HMGI/Y proteins: An immunocytochemical study
RICCIO, Massimo;
1998
Abstract
The intranuclear distribution of HMGI/Y proteins was analyzed by immunofluorescent staining in several cell lines using a polyclonal antibody that stained a fibrogranular network. In actively growing 3T3 fibroblasts, HMGI/Y proteins were mainly localized to heterochromatin masses, whereas in quiescent cells they were more diffusely distributed. Double labeling experiments showed a co-localization of HMGI/Y with DNA topoisomerase II alpha. These results are in agreement with previously published biochemical data and indicate a possible involvement of HMGI/Y proteins in several nuclear functions, including chromatin organization and gene expression.
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