The dimorphic transition of Candida albicans from the yeast (Y-Candida) to the hyphal (H-Candida) form is a complex event; the relevance of this transition in fungal pathogenicity is still poorly understood. By using a cloned macrophage cell line (ANA-1), we questioned whether the interaction between macrophages and Y-Candida or H-Candida could affect specific cell functions, i.e., tumor necrosis factor and lysozyme production. We found that ANA-1 macrophages selectively responded to H-Candida with increased tumor necrosis factor and downregulated lysozyme, as assessed by measurement of relative mRNA levels and secreted biological activities. The H-Candida-mediated effects were (i) dependent upon the ratio between ANA-1 macrophages and H-Candida, (ii) detectable after 1 h of coincubation, and (iii) accomplished without fungal ingestion. Conversely, Y-Candida, which was found inside the ANA-1 macrophages, did not affect tumor necrosis factor and lysozyme production, nor did it prevent the macrophage response to other stimuli. Overall, these results indicate that a macrophage can distinguish between Y-Candida and H-Candida and that only the latter is able to modulate specific functions. H-Candida is recognized and probably processed as an extracellular target. The possible implication of macrophages as autocrine and paracrine regulatory cells during Candida infections is discussed.

Early Differential Molecular Response of a Macrophage Cell Line toYeast and Hyphal Forms of Candida albicans / Blasi, Elisabetta; L., Pitzurra; M., Puliti; L., Lanfrancone; F., Bistoni. - In: INFECTION AND IMMUNITY. - ISSN 0019-9567. - ELETTRONICO. - 60(3):(1992), pp. 832-837.

Early Differential Molecular Response of a Macrophage Cell Line toYeast and Hyphal Forms of Candida albicans

BLASI, Elisabetta;
1992

Abstract

The dimorphic transition of Candida albicans from the yeast (Y-Candida) to the hyphal (H-Candida) form is a complex event; the relevance of this transition in fungal pathogenicity is still poorly understood. By using a cloned macrophage cell line (ANA-1), we questioned whether the interaction between macrophages and Y-Candida or H-Candida could affect specific cell functions, i.e., tumor necrosis factor and lysozyme production. We found that ANA-1 macrophages selectively responded to H-Candida with increased tumor necrosis factor and downregulated lysozyme, as assessed by measurement of relative mRNA levels and secreted biological activities. The H-Candida-mediated effects were (i) dependent upon the ratio between ANA-1 macrophages and H-Candida, (ii) detectable after 1 h of coincubation, and (iii) accomplished without fungal ingestion. Conversely, Y-Candida, which was found inside the ANA-1 macrophages, did not affect tumor necrosis factor and lysozyme production, nor did it prevent the macrophage response to other stimuli. Overall, these results indicate that a macrophage can distinguish between Y-Candida and H-Candida and that only the latter is able to modulate specific functions. H-Candida is recognized and probably processed as an extracellular target. The possible implication of macrophages as autocrine and paracrine regulatory cells during Candida infections is discussed.
1992
60(3)
832
837
Early Differential Molecular Response of a Macrophage Cell Line toYeast and Hyphal Forms of Candida albicans / Blasi, Elisabetta; L., Pitzurra; M., Puliti; L., Lanfrancone; F., Bistoni. - In: INFECTION AND IMMUNITY. - ISSN 0019-9567. - ELETTRONICO. - 60(3):(1992), pp. 832-837.
Blasi, Elisabetta; L., Pitzurra; M., Puliti; L., Lanfrancone; F., Bistoni
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11380/776305
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