We have established a new human plasma cell line from the peripheral blood of a patient with an IgA-kappa plasma-cell leukemia. Morphological, immunological, cytogenetic and molecular studies confirm that the cultured cells are derived from the same clone of leukemic plasma cell in vivo. The established cell line (MT3) grows in suspension, secretes high amounts of IgA kappa and exhibits morphological and ultrastructural characteristics of plasma cells. Surface marker analysis shows that both primary and cultured cells express the plasma-cell-associated antigens PCA-1 and T10, while specific B- and T-cell determinants and EBV nuclear antigen are undetectable. In the established cell line a few cells express Ia-like and CALLA antigens. Cytogenetic analysis of MT3 cells reveals a prevalent hypertriploid karyotype with constant chromosomal aberrations consisting of 14q+, 22q- and marker chromosomes

Establishment and characterization of a human IgA-kappa-secreting plasma cell line (MT3) / Donelli, A; Narni, Franco; Tabilio, A; Emilia, Giovanni; Selleri, L; Colo, A; Zucchini, Patrizia; Montagnani, Giuliano; Torelli, Giuseppe; Torelli, Umberto. - In: INTERNATIONAL JOURNAL OF CANCER. - ISSN 0020-7136. - STAMPA. - 40:(1987), pp. 383-388.

Establishment and characterization of a human IgA-kappa-secreting plasma cell line (MT3).

NARNI, Franco;EMILIA, Giovanni;ZUCCHINI, Patrizia;MONTAGNANI, Giuliano;TORELLI, Giuseppe;TORELLI, Umberto
1987

Abstract

We have established a new human plasma cell line from the peripheral blood of a patient with an IgA-kappa plasma-cell leukemia. Morphological, immunological, cytogenetic and molecular studies confirm that the cultured cells are derived from the same clone of leukemic plasma cell in vivo. The established cell line (MT3) grows in suspension, secretes high amounts of IgA kappa and exhibits morphological and ultrastructural characteristics of plasma cells. Surface marker analysis shows that both primary and cultured cells express the plasma-cell-associated antigens PCA-1 and T10, while specific B- and T-cell determinants and EBV nuclear antigen are undetectable. In the established cell line a few cells express Ia-like and CALLA antigens. Cytogenetic analysis of MT3 cells reveals a prevalent hypertriploid karyotype with constant chromosomal aberrations consisting of 14q+, 22q- and marker chromosomes
40
383
388
Establishment and characterization of a human IgA-kappa-secreting plasma cell line (MT3) / Donelli, A; Narni, Franco; Tabilio, A; Emilia, Giovanni; Selleri, L; Colo, A; Zucchini, Patrizia; Montagnani, Giuliano; Torelli, Giuseppe; Torelli, Umberto. - In: INTERNATIONAL JOURNAL OF CANCER. - ISSN 0020-7136. - STAMPA. - 40:(1987), pp. 383-388.
Donelli, A; Narni, Franco; Tabilio, A; Emilia, Giovanni; Selleri, L; Colo, A; Zucchini, Patrizia; Montagnani, Giuliano; Torelli, Giuseppe; Torelli, Umberto
File in questo prodotto:
Non ci sono file associati a questo prodotto.
Pubblicazioni consigliate

Caricamento pubblicazioni consigliate

Licenza Creative Commons
I metadati presenti in IRIS UNIMORE sono rilasciati con licenza Creative Commons CC0 1.0 Universal, mentre i file delle pubblicazioni sono rilasciati con licenza Attribuzione 4.0 Internazionale (CC BY 4.0), salvo diversa indicazione.
In caso di violazione di copyright, contattare Supporto Iris

Utilizza questo identificativo per citare o creare un link a questo documento: http://hdl.handle.net/11380/746666
Citazioni
  • ???jsp.display-item.citation.pmc??? 0
  • Scopus 14
  • ???jsp.display-item.citation.isi??? 17
social impact