Time course and "chase" experiments showed that, after incubation of acute myeloid leukemia blast cells with a labeled RNA precursor, a large proportion of radioactivity remained associated with RNA molecules larger than 45 S even after several hr. Double-labeling experiments with [5-3H]uridine and [methyl-14C]methionine indicated that unmethylated giant heterogeneous RNA larger than 45 S is processed much more slowly than the 45 S ribosomal precursor, so that relatively large amounts of fairly stable RNA of the former class accumulate in the cell. The measurement of labeled giant heterogeneous RNA molecules bound to polyuridylate-fiberglass filters showed that molecules carrying polyadenylate segments seemingly turn over faster than those lacking polyadenylate.
Accumulation of giant heterogeneous RNA molecules in acute myeloid leukemia blast cells / Torelli, Umberto; Torelli, Giuseppe; Cadossi, Ruggero; Ferrari, Stefano; Ferrari, Sergio; Narni, Franco; Montagnani, Giuliano. - In: CANCER RESEARCH. - ISSN 0008-5472. - STAMPA. - 36:(1976), pp. 4631-4638.
Accumulation of giant heterogeneous RNA molecules in acute myeloid leukemia blast cells.
TORELLI, Umberto;TORELLI, Giuseppe;CADOSSI, Ruggero;FERRARI, Stefano;FERRARI, Sergio;NARNI, Franco;MONTAGNANI, Giuliano
1976
Abstract
Time course and "chase" experiments showed that, after incubation of acute myeloid leukemia blast cells with a labeled RNA precursor, a large proportion of radioactivity remained associated with RNA molecules larger than 45 S even after several hr. Double-labeling experiments with [5-3H]uridine and [methyl-14C]methionine indicated that unmethylated giant heterogeneous RNA larger than 45 S is processed much more slowly than the 45 S ribosomal precursor, so that relatively large amounts of fairly stable RNA of the former class accumulate in the cell. The measurement of labeled giant heterogeneous RNA molecules bound to polyuridylate-fiberglass filters showed that molecules carrying polyadenylate segments seemingly turn over faster than those lacking polyadenylate.Pubblicazioni consigliate
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