Time course and "chase" experiments showed that, after incubation of acute myeloid leukemia blast cells with a labeled RNA precursor, a large proportion of radioactivity remained associated with RNA molecules larger than 45 S even after several hr. Double-labeling experiments with [5-3H]uridine and [methyl-14C]methionine indicated that unmethylated giant heterogeneous RNA larger than 45 S is processed much more slowly than the 45 S ribosomal precursor, so that relatively large amounts of fairly stable RNA of the former class accumulate in the cell. The measurement of labeled giant heterogeneous RNA molecules bound to polyuridylate-fiberglass filters showed that molecules carrying polyadenylate segments seemingly turn over faster than those lacking polyadenylate.

Accumulation of giant heterogeneous RNA molecules in acute myeloid leukemia blast cells / Torelli, Umberto; Torelli, Giuseppe; Cadossi, Ruggero; Ferrari, Stefano; Ferrari, Sergio; Narni, Franco; Montagnani, Giuliano. - In: CANCER RESEARCH. - ISSN 0008-5472. - STAMPA. - 36:(1976), pp. 4631-4638.

Accumulation of giant heterogeneous RNA molecules in acute myeloid leukemia blast cells.

TORELLI, Umberto;TORELLI, Giuseppe;CADOSSI, Ruggero;FERRARI, Stefano;FERRARI, Sergio;NARNI, Franco;MONTAGNANI, Giuliano
1976

Abstract

Time course and "chase" experiments showed that, after incubation of acute myeloid leukemia blast cells with a labeled RNA precursor, a large proportion of radioactivity remained associated with RNA molecules larger than 45 S even after several hr. Double-labeling experiments with [5-3H]uridine and [methyl-14C]methionine indicated that unmethylated giant heterogeneous RNA larger than 45 S is processed much more slowly than the 45 S ribosomal precursor, so that relatively large amounts of fairly stable RNA of the former class accumulate in the cell. The measurement of labeled giant heterogeneous RNA molecules bound to polyuridylate-fiberglass filters showed that molecules carrying polyadenylate segments seemingly turn over faster than those lacking polyadenylate.
36
4631
4638
Accumulation of giant heterogeneous RNA molecules in acute myeloid leukemia blast cells / Torelli, Umberto; Torelli, Giuseppe; Cadossi, Ruggero; Ferrari, Stefano; Ferrari, Sergio; Narni, Franco; Montagnani, Giuliano. - In: CANCER RESEARCH. - ISSN 0008-5472. - STAMPA. - 36:(1976), pp. 4631-4638.
Torelli, Umberto; Torelli, Giuseppe; Cadossi, Ruggero; Ferrari, Stefano; Ferrari, Sergio; Narni, Franco; Montagnani, Giuliano
File in questo prodotto:
Non ci sono file associati a questo prodotto.
Pubblicazioni consigliate

Caricamento pubblicazioni consigliate

Licenza Creative Commons
I metadati presenti in IRIS UNIMORE sono rilasciati con licenza Creative Commons CC0 1.0 Universal, mentre i file delle pubblicazioni sono rilasciati con licenza Attribuzione 4.0 Internazionale (CC BY 4.0), salvo diversa indicazione.
In caso di violazione di copyright, contattare Supporto Iris

Utilizza questo identificativo per citare o creare un link a questo documento: http://hdl.handle.net/11380/746465
Citazioni
  • ???jsp.display-item.citation.pmc??? 0
  • Scopus 19
  • ???jsp.display-item.citation.isi??? 25
social impact