A murine cell line (BV-2) has been generated by infecting primary microglial cell cultures with a v-raf/v-myc oncogene carrying retrovirus (J2). BV-2 cells expressed nonspecific esterase activity, phagocytic ability and lacked peroxidase activity. Such cells secreted lysozyme and, following appropriate stimulation, also interleukin 1 and tumor necrosis factor. Furthermore, BV-2 cells exhibited spontaneous anti-Candida activity and acquired tumoricidal activity upon treatment with interferon-gamma. Phenotypically, BV-2 cells resulted positive for MAC1 and MAC2 antigens, and negative for MAC3, glial fibrillary acidic protein (GFAP) and galactocerebroside (GC) antigens. Since BV-2 cells retain most of the morphological, phenotypical and functional properties described for freshly isolated microglial cells, we can conclude that J2 virus infection has resulted in the immortalization of active microglial cells

Immortalization of murine microglial cells by a v-raf/v-myc carrying retrovirus / Blasi, Elisabetta; Barluzzi, R.; Bocchini, V.; Mazzolla, R.; Bistoni, F.. - In: JOURNAL OF NEUROIMMUNOLOGY. - ISSN 0165-5728. - ELETTRONICO. - 27:(1990), pp. 229-237.

Immortalization of murine microglial cells by a v-raf/v-myc carrying retrovirus.

BLASI, Elisabetta;
1990-01-01

Abstract

A murine cell line (BV-2) has been generated by infecting primary microglial cell cultures with a v-raf/v-myc oncogene carrying retrovirus (J2). BV-2 cells expressed nonspecific esterase activity, phagocytic ability and lacked peroxidase activity. Such cells secreted lysozyme and, following appropriate stimulation, also interleukin 1 and tumor necrosis factor. Furthermore, BV-2 cells exhibited spontaneous anti-Candida activity and acquired tumoricidal activity upon treatment with interferon-gamma. Phenotypically, BV-2 cells resulted positive for MAC1 and MAC2 antigens, and negative for MAC3, glial fibrillary acidic protein (GFAP) and galactocerebroside (GC) antigens. Since BV-2 cells retain most of the morphological, phenotypical and functional properties described for freshly isolated microglial cells, we can conclude that J2 virus infection has resulted in the immortalization of active microglial cells
27
229
237
Immortalization of murine microglial cells by a v-raf/v-myc carrying retrovirus / Blasi, Elisabetta; Barluzzi, R.; Bocchini, V.; Mazzolla, R.; Bistoni, F.. - In: JOURNAL OF NEUROIMMUNOLOGY. - ISSN 0165-5728. - ELETTRONICO. - 27:(1990), pp. 229-237.
Blasi, Elisabetta; Barluzzi, R.; Bocchini, V.; Mazzolla, R.; Bistoni, F.
File in questo prodotto:
Non ci sono file associati a questo prodotto.
Pubblicazioni consigliate

Licenza Creative Commons
I metadati presenti in IRIS UNIMORE sono rilasciati con licenza Creative Commons CC0 1.0 Universal, mentre i file delle pubblicazioni sono rilasciati con licenza Attribuzione 4.0 Internazionale (CC BY 4.0), salvo diversa indicazione.
In caso di violazione di copyright, contattare Supporto Iris

Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11380/746006
Citazioni
  • ???jsp.display-item.citation.pmc??? 334
  • Scopus 820
  • ???jsp.display-item.citation.isi??? 824
social impact