Nine universal cpSSR markers were tested on a representative group of cultivated accessions and spontaneous ribespopulations. All primer combinations, with the exception of ccmp8, underwent amplification. Monomorphic alleles weredetected at the ccmp2, ccmp3, ccmp4, ccmp5, ccmp7, ccmp9 primer pairs. Two and four polymorphic alleles were observedat the ccmp6 and ccmp10 loci, respectively. These six alleles combined in five different haplotypes, two of which were foundin the cultivated accessions, and three only in the spontaneous populations. AFLP analysis was also performed to betterdefine the relationships among cultivated and spontaneous varieties, and to compare nuclear markers with chloroplastmicrosatellites. AFLP data were able to distinguish clearly all cultivars, while in spontaneous populations the detectedpolymorphisms were too low. In conclusion, cpSSR markers can be considered useful in the characterisation of ribesaccessions. Thus, the combination of this tool with nuclear markers, such as AFLP, could help in distinguishing andcharacterising each cultivated accession, and in defining population genetic parameters in the study of spontaneous Ribesspecies

Chloroplast and nuclear DNA markers to characterize cultivated and spontaneous Ribes / F., De Mattia; F., Grassi; Imazio, Serena Anna; M., Labra. - In: PLANT BIOSYSTEMS. - ISSN 1724-5575. - ELETTRONICO. - 142:2(2008), pp. 204-212. [10.1080/11263500802150290]

Chloroplast and nuclear DNA markers to characterize cultivated and spontaneous Ribes

IMAZIO, Serena Anna;
2008

Abstract

Nine universal cpSSR markers were tested on a representative group of cultivated accessions and spontaneous ribespopulations. All primer combinations, with the exception of ccmp8, underwent amplification. Monomorphic alleles weredetected at the ccmp2, ccmp3, ccmp4, ccmp5, ccmp7, ccmp9 primer pairs. Two and four polymorphic alleles were observedat the ccmp6 and ccmp10 loci, respectively. These six alleles combined in five different haplotypes, two of which were foundin the cultivated accessions, and three only in the spontaneous populations. AFLP analysis was also performed to betterdefine the relationships among cultivated and spontaneous varieties, and to compare nuclear markers with chloroplastmicrosatellites. AFLP data were able to distinguish clearly all cultivars, while in spontaneous populations the detectedpolymorphisms were too low. In conclusion, cpSSR markers can be considered useful in the characterisation of ribesaccessions. Thus, the combination of this tool with nuclear markers, such as AFLP, could help in distinguishing andcharacterising each cultivated accession, and in defining population genetic parameters in the study of spontaneous Ribesspecies
2008
142
2
204
212
Chloroplast and nuclear DNA markers to characterize cultivated and spontaneous Ribes / F., De Mattia; F., Grassi; Imazio, Serena Anna; M., Labra. - In: PLANT BIOSYSTEMS. - ISSN 1724-5575. - ELETTRONICO. - 142:2(2008), pp. 204-212. [10.1080/11263500802150290]
F., De Mattia; F., Grassi; Imazio, Serena Anna; M., Labra
File in questo prodotto:
File Dimensione Formato  
De Mattia_2008_Plantbiosystems.pdf

Accesso riservato

Tipologia: Versione dell'autore revisionata e accettata per la pubblicazione
Dimensione 428.7 kB
Formato Adobe PDF
428.7 kB Adobe PDF   Visualizza/Apri   Richiedi una copia
Pubblicazioni consigliate

Licenza Creative Commons
I metadati presenti in IRIS UNIMORE sono rilasciati con licenza Creative Commons CC0 1.0 Universal, mentre i file delle pubblicazioni sono rilasciati con licenza Attribuzione 4.0 Internazionale (CC BY 4.0), salvo diversa indicazione.
In caso di violazione di copyright, contattare Supporto Iris

Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11380/744479
Citazioni
  • ???jsp.display-item.citation.pmc??? ND
  • Scopus 19
  • ???jsp.display-item.citation.isi??? 18
social impact