The cytotoxic activity of human recombinant tumor necrosis factor (rHuTNF) (from 0.01 to 10000 U/ml) was assayed on six human ovarian cancer cell lines and one human cervical carcinoma cell line using a crystal violet assay. rHuTNF was cytotoxic to four cell lines (A2780, A2774, SW626, PA1), while 3 cell lines (IGROV1, SKOV3, Me180) were marginally sensitive to its activity. However, under the same experimental conditions rHuTNF markedly enhanced the cytotoxicity of mitoxantrone, a chemotherapeutic drug targeted at DNA topoisomerase II, in six cell lines. The potentiation of mitoxantrone cytotoxicity was not caused by increased drug accumulation after rHuTNF treatment. No significant increase in cytotoxicity to Me180 cell line was seen when rHuTNF was added to mitoxantrone.

Potentiation by tumor necrosis factor of mitoxantrone cytotoxicity to human ovarian cancer cell lines / P., Russo; S., Parodi; G., Billi; C., Oliva; M., Venturini; E., Noviello; Conte, Pierfranco. - In: JAPANESE JOURNAL OF CANCER RESEARCH. - ISSN 0910-5050. - STAMPA. - 83:(1992), pp. 684-687.

Potentiation by tumor necrosis factor of mitoxantrone cytotoxicity to human ovarian cancer cell lines.

CONTE, Pierfranco
1992

Abstract

The cytotoxic activity of human recombinant tumor necrosis factor (rHuTNF) (from 0.01 to 10000 U/ml) was assayed on six human ovarian cancer cell lines and one human cervical carcinoma cell line using a crystal violet assay. rHuTNF was cytotoxic to four cell lines (A2780, A2774, SW626, PA1), while 3 cell lines (IGROV1, SKOV3, Me180) were marginally sensitive to its activity. However, under the same experimental conditions rHuTNF markedly enhanced the cytotoxicity of mitoxantrone, a chemotherapeutic drug targeted at DNA topoisomerase II, in six cell lines. The potentiation of mitoxantrone cytotoxicity was not caused by increased drug accumulation after rHuTNF treatment. No significant increase in cytotoxicity to Me180 cell line was seen when rHuTNF was added to mitoxantrone.
1992
83
684
687
Potentiation by tumor necrosis factor of mitoxantrone cytotoxicity to human ovarian cancer cell lines / P., Russo; S., Parodi; G., Billi; C., Oliva; M., Venturini; E., Noviello; Conte, Pierfranco. - In: JAPANESE JOURNAL OF CANCER RESEARCH. - ISSN 0910-5050. - STAMPA. - 83:(1992), pp. 684-687.
P., Russo; S., Parodi; G., Billi; C., Oliva; M., Venturini; E., Noviello; Conte, Pierfranco
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11380/739564
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