In spite of their apparently restricted differentiation potentiality, hematopoietic precursors are plastic cells able to trans-differentiate from a maturation lineage to another. To better characterize this differentiation plasticity, we purified CD14- and CD14+ myeloid precursors generated by 'in vitro' culture of human CD34+ hematopoietic progenitors. Morphological analysis of the investigated cell populations indicated that, as expected, they consisted of granulocyte and monocyte precursors, respectively. Treatment with differentiation inducers revealed that CD14- cells were bipotent granulo-monocyte precursors, while CD14+ cells appeared univocally committed to a terminal macrophage maturation. Flow cytometry analysis demonstrated that the conversion of granulocyte precursors to the mono-macrophage maturation lineage occurs through a differentiation transition in which the granulocyte-related myeloperoxidase enzyme and the monocyte-specific CD14 antigen are co-expressed. Expression profiling evidenced that the observed trans-differentiation process was accompanied by a remarkable upregulation of the monocyte-related MafB transcription factor.
Correlation between differentiation plasticity and mRNA expression profiling of CD34+-derived CD14- and CD14+ human normal myeloid precursors / MONTANARI, Monica; GEMELLI, Claudia; TENEDINI, Elena; ZANOCCO MARANI, Tommaso; Vignudelli, T; Siena, M; ZINI, Roberta; SALATI, Simona; Chiossi, G; TAGLIAFICO, Enrico; MANFREDINI, Rossella; GRANDE, Alexis; FERRARI, Sergio. - In: CELL DEATH AND DIFFERENTIATION. - ISSN 1350-9047. - STAMPA. - 12(2005), pp. 1588-1600.
Data di pubblicazione: | 2005 |
Titolo: | Correlation between differentiation plasticity and mRNA expression profiling of CD34+-derived CD14- and CD14+ human normal myeloid precursors |
Autore/i: | MONTANARI, Monica; GEMELLI, Claudia; TENEDINI, Elena; ZANOCCO MARANI, Tommaso; Vignudelli, T; Siena, M; ZINI, Roberta; SALATI, Simona; Chiossi, G; TAGLIAFICO, Enrico; MANFREDINI, Rossella; GRANDE, Alexis; FERRARI, Sergio |
Autore/i UNIMORE: | |
Digital Object Identifier (DOI): | http://dx.doi.org/10.1038/sj.cdd.4401679 |
Rivista: | |
Volume: | 12 |
Pagina iniziale: | 1588 |
Pagina finale: | 1600 |
Codice identificativo ISI: | WOS:000233169200005 |
Codice identificativo Scopus: | 2-s2.0-27944456492 |
Codice identificativo Pubmed: | 15947790 |
Citazione: | Correlation between differentiation plasticity and mRNA expression profiling of CD34+-derived CD14- and CD14+ human normal myeloid precursors / MONTANARI, Monica; GEMELLI, Claudia; TENEDINI, Elena; ZANOCCO MARANI, Tommaso; Vignudelli, T; Siena, M; ZINI, Roberta; SALATI, Simona; Chiossi, G; TAGLIAFICO, Enrico; MANFREDINI, Rossella; GRANDE, Alexis; FERRARI, Sergio. - In: CELL DEATH AND DIFFERENTIATION. - ISSN 1350-9047. - STAMPA. - 12(2005), pp. 1588-1600. |
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