The enantioseparation of reboxetine by HPLC was investigated using chiral stationary phases (CSPs) containingcellulose Tris(3,5-dimethylphenyl)carbamate on silica gel (Chiralcel OD column) as the chiralselector. Reversed phase HPLC was the technique of choice for the analytical enantioseparation of reboxetine,while the chiral semipreparative separation was obtained with the same CSP, but in normal phaseconditions. The effects of the mobile phase pH and composition on analytical retention, enantioselectivityand resolution were investigated. The best performance was obtained using a mobile phase composed of0.5Msodium perchlorate at pH 6 and acetonitrile in the 60/40 (v/v) ratio. The semipreparative separationhas allowed obtaining pure enantiomers, but required the preparation of reboxetine free base. Differentn-hexane/alcohol mixtures were tested as mobile phases, varying both the nature of the alcohol and itspercentage in the mobile phase. Different n-hexane/alcohol mixtures were tested as mobile phase andthe best results were obtained by using a mobile phase composed of n-hexane and 2-propanol (80:20,v/v).

Enantioseparation of the antidepressant reboxetine / Cannazza, Giuseppe; Braghiroli, Daniela; Carrozzo, Marina Maria; Parenti, Carlo; Sabbioni, Cesare; Mandrioli, Roberto; Fanali, Salvatore; Raggi, Maria Augusta. - In: JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS. - ISSN 0731-7085. - STAMPA. - 48(2008), pp. 991-996. [10.1016/j.jpba.2008.06.026]

Enantioseparation of the antidepressant reboxetine

CANNAZZA, Giuseppe;BRAGHIROLI, Daniela;CARROZZO, Marina Maria;PARENTI, Carlo;
2008

Abstract

The enantioseparation of reboxetine by HPLC was investigated using chiral stationary phases (CSPs) containingcellulose Tris(3,5-dimethylphenyl)carbamate on silica gel (Chiralcel OD column) as the chiralselector. Reversed phase HPLC was the technique of choice for the analytical enantioseparation of reboxetine,while the chiral semipreparative separation was obtained with the same CSP, but in normal phaseconditions. The effects of the mobile phase pH and composition on analytical retention, enantioselectivityand resolution were investigated. The best performance was obtained using a mobile phase composed of0.5Msodium perchlorate at pH 6 and acetonitrile in the 60/40 (v/v) ratio. The semipreparative separationhas allowed obtaining pure enantiomers, but required the preparation of reboxetine free base. Differentn-hexane/alcohol mixtures were tested as mobile phases, varying both the nature of the alcohol and itspercentage in the mobile phase. Different n-hexane/alcohol mixtures were tested as mobile phase andthe best results were obtained by using a mobile phase composed of n-hexane and 2-propanol (80:20,v/v).
48
991
996
Enantioseparation of the antidepressant reboxetine / Cannazza, Giuseppe; Braghiroli, Daniela; Carrozzo, Marina Maria; Parenti, Carlo; Sabbioni, Cesare; Mandrioli, Roberto; Fanali, Salvatore; Raggi, Maria Augusta. - In: JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS. - ISSN 0731-7085. - STAMPA. - 48(2008), pp. 991-996. [10.1016/j.jpba.2008.06.026]
Cannazza, Giuseppe; Braghiroli, Daniela; Carrozzo, Marina Maria; Parenti, Carlo; Sabbioni, Cesare; Mandrioli, Roberto; Fanali, Salvatore; Raggi, Maria Augusta
File in questo prodotto:
File Dimensione Formato  
Enantioseparation of the antidepressant reboxetine.pdf

non disponibili

Tipologia: Versione dell'editore (versione pubblicata)
Dimensione 352.39 kB
Formato Adobe PDF
352.39 kB Adobe PDF   Visualizza/Apri   Richiedi una copia
Pubblicazioni consigliate

Caricamento pubblicazioni consigliate

Licenza Creative Commons
I metadati presenti in IRIS UNIMORE sono rilasciati con licenza Creative Commons CC0 1.0 Universal, mentre i file delle pubblicazioni sono rilasciati con licenza Attribuzione 4.0 Internazionale (CC BY 4.0), salvo diversa indicazione.
In caso di violazione di copyright, contattare Supporto Iris

Utilizza questo identificativo per citare o creare un link a questo documento: http://hdl.handle.net/11380/715849
Citazioni
  • ???jsp.display-item.citation.pmc??? 0
  • Scopus 7
  • ???jsp.display-item.citation.isi??? 6
social impact