The tuberculin skin test (TST) does not discriminate between recent and remote latent tuberculosis infection (LTBI). This study was carried out to test two interferon-gamma-based blood assays in recent contacts with high prevalence of remote LTBI. We performed a contact tracing investigation in a nursing home for the elderly, where elderly patients were exposed to a case of pulmonary tuberculosis. TST, QuantiFERON-TB Gold (QFT-G) and T-SPOT.TB (TS.TB) were performed 8 weeks after the end of potential exposure. IFN-gamma measurements were recorded and correlation with exposure was evaluated. Twenty-seven (37.5%), 32 (44.4%) and 16 (22.2%) subjects were TST, TS.TB and QFT-G positive, respectively; agreement between TS.TB and QFT-G was good among exposed subjects only (K=0.915, 0.218 in unexposed, p<0.001). When amounts of IFN-gamma were corrected for the number of producing T cells, specific IFN-gamma production was significantly different between exposed and unexposed individuals (16.75+/-5.40 vs 2.33+/-0.71 IFN-gamma IU/1000 SFC, p=0.0001). QFT-G and TS.TB provided discordant results among elderly contacts. Unlike TST, the specific IFN-gamma response might discriminate between recent and long-lasting tuberculosis infection.

Interferon-gamma-release assays detect tuberculosis re-infection in elderly contacts / G., Ferrara; M., Losi; D'Amico, Roberto; R., Cagarelli; Am, Pezzi; M., Meacci; B., Meccugni; I., Dori Marchetti; F., Rumpianesi; P., Roversi; L., Casali; Lm, Fabbri; Richeldi, Luca. - In: INTERNATIONAL JOURNAL OF IMMUNOPATHOLOGY AND PHARMACOLOGY. - ISSN 0394-6320. - STAMPA. - 22:3(2009), pp. 669-677. [10.1177/039463200902200312]

Interferon-gamma-release assays detect tuberculosis re-infection in elderly contacts

D'AMICO, Roberto;RICHELDI, Luca
2009

Abstract

The tuberculin skin test (TST) does not discriminate between recent and remote latent tuberculosis infection (LTBI). This study was carried out to test two interferon-gamma-based blood assays in recent contacts with high prevalence of remote LTBI. We performed a contact tracing investigation in a nursing home for the elderly, where elderly patients were exposed to a case of pulmonary tuberculosis. TST, QuantiFERON-TB Gold (QFT-G) and T-SPOT.TB (TS.TB) were performed 8 weeks after the end of potential exposure. IFN-gamma measurements were recorded and correlation with exposure was evaluated. Twenty-seven (37.5%), 32 (44.4%) and 16 (22.2%) subjects were TST, TS.TB and QFT-G positive, respectively; agreement between TS.TB and QFT-G was good among exposed subjects only (K=0.915, 0.218 in unexposed, p<0.001). When amounts of IFN-gamma were corrected for the number of producing T cells, specific IFN-gamma production was significantly different between exposed and unexposed individuals (16.75+/-5.40 vs 2.33+/-0.71 IFN-gamma IU/1000 SFC, p=0.0001). QFT-G and TS.TB provided discordant results among elderly contacts. Unlike TST, the specific IFN-gamma response might discriminate between recent and long-lasting tuberculosis infection.
2009
22
3
669
677
Interferon-gamma-release assays detect tuberculosis re-infection in elderly contacts / G., Ferrara; M., Losi; D'Amico, Roberto; R., Cagarelli; Am, Pezzi; M., Meacci; B., Meccugni; I., Dori Marchetti; F., Rumpianesi; P., Roversi; L., Casali; Lm, Fabbri; Richeldi, Luca. - In: INTERNATIONAL JOURNAL OF IMMUNOPATHOLOGY AND PHARMACOLOGY. - ISSN 0394-6320. - STAMPA. - 22:3(2009), pp. 669-677. [10.1177/039463200902200312]
G., Ferrara; M., Losi; D'Amico, Roberto; R., Cagarelli; Am, Pezzi; M., Meacci; B., Meccugni; I., Dori Marchetti; F., Rumpianesi; P., Roversi; L., Casali; Lm, Fabbri; Richeldi, Luca
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11380/710998
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