University of Wisconsin solution (U.W.S.) has prolonged hypotermic preservation of liver and kidney. This solution has been afterwards tested for heart and lung preservation. The high-potassium content and viscosity of the solution suggested to us to bring some modifications: addition of plasma expanders, aprotinin and an increased concentration of gluthatione. We studied the effect of this original solution using an animal model: 10 males adult Yuchatan micro-pig, undergoing excision of the heart-lung block. A morphological study of preserved lung was performed, then we compared the effect of standard UW solution and our modified solution. The cyto-histological findings, at different preservation times, showed that superior results have been obtained with the modified UW solution, such as better hematic wash-out from the pulmonary vessels and milder vasoconstriction in the pulmonary artery bed. Using our solution we observed a satisfactory preservation of pulmonary endothelium and bronchial epithelium respectively after 8 and 12 hours of hypotermic ischemia. This was the first step of our research; in a further study we shall perform the single-lung transplantation in a micro-pig model, to report a functional in vivo evaluation of the clinical applicability and quality of our solution for lung preservation

Morphological researches on lung preservation with a modified University of Wisconsin solution containing gelatine polypeptides (Haemaccel) / G., Spagna; P., Bruzzone; I., Pecorella; R., Lorenzini; Stefani, Alessandro; Morandi, Uliano; R., Lodi. - In: ARCHIVIO DI CHIRURGIA TORACICA E CARDIOVASCOLARE. - ISSN 0391-7029. - STAMPA. - 19:(1997), pp. 113-120.

Morphological researches on lung preservation with a modified University of Wisconsin solution containing gelatine polypeptides (Haemaccel)

STEFANI, Alessandro;MORANDI, Uliano;
1997

Abstract

University of Wisconsin solution (U.W.S.) has prolonged hypotermic preservation of liver and kidney. This solution has been afterwards tested for heart and lung preservation. The high-potassium content and viscosity of the solution suggested to us to bring some modifications: addition of plasma expanders, aprotinin and an increased concentration of gluthatione. We studied the effect of this original solution using an animal model: 10 males adult Yuchatan micro-pig, undergoing excision of the heart-lung block. A morphological study of preserved lung was performed, then we compared the effect of standard UW solution and our modified solution. The cyto-histological findings, at different preservation times, showed that superior results have been obtained with the modified UW solution, such as better hematic wash-out from the pulmonary vessels and milder vasoconstriction in the pulmonary artery bed. Using our solution we observed a satisfactory preservation of pulmonary endothelium and bronchial epithelium respectively after 8 and 12 hours of hypotermic ischemia. This was the first step of our research; in a further study we shall perform the single-lung transplantation in a micro-pig model, to report a functional in vivo evaluation of the clinical applicability and quality of our solution for lung preservation
19
113
120
Morphological researches on lung preservation with a modified University of Wisconsin solution containing gelatine polypeptides (Haemaccel) / G., Spagna; P., Bruzzone; I., Pecorella; R., Lorenzini; Stefani, Alessandro; Morandi, Uliano; R., Lodi. - In: ARCHIVIO DI CHIRURGIA TORACICA E CARDIOVASCOLARE. - ISSN 0391-7029. - STAMPA. - 19:(1997), pp. 113-120.
G., Spagna; P., Bruzzone; I., Pecorella; R., Lorenzini; Stefani, Alessandro; Morandi, Uliano; R., Lodi
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11380/708797
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