In the present work, we analyzed the survival features of six different Epstein–Barr virus (EBV)-stabilized lymphoid cell lines obtained from adult subjects and from subjects of more than 95 years. For the first, we found that lymphoid B cells from centenarians were more resistant to apoptosis induction and displayed a more developed lysosomal compartment, the most critical component of phagic machinery, in comparison with lymphoid B cells from adult subjects. In addition, cells from centenarians were capable of engulfing and digesting other cells, i.e., their siblings (even entire cells), whereas lymphoid cells from “control samples”, i.e., from adults, did not. This behavior was improved by nutrient deprivation but, strikingly, it was unaffected by the autophagy-modulating drug, rapamycin, an autophagy inducer, and 3-methyladenine, an autophagy inhibitor. Transcriptomic analyses indicated that: (1) aspartyl proteases, (2) cell surface molecules such as integrins and cadherins, and (3) some components of cytoskeletal network could contribute to establish this survival phenotype. Also, Kyoto Encyclopedia of Genes and Genomes pathways such as Wnt signaling pathway, an essential contributor to cell migration and actin cytoskeleton remodeling, appeared as prominent. Although we cannot rule out the possibility that EBV-immortalization could play a role, since we observed this phagic behavior in cells from centenarians but not in those from adults, we hypothesize that it may represent an important survival determinant in cells from centenarians.

Survival features of EBV-stabilized cells from centenarians: morpho-functional and transcriptomic analyses / Matarrese, P; Tinari, A; Ascione, B; Gambardella, L; Remondini, D; Salvioli, S; Tenedini, Elena; Tagliafico, Enrico; Franceschi, C; Malorni, W.. - In: AGE. - ISSN 1574-4647. - STAMPA. - 34:6(2012), pp. 1341-1359. [10.1007/s11357-011-9307-4]

Survival features of EBV-stabilized cells from centenarians: morpho-functional and transcriptomic analyses.

TENEDINI, Elena;TAGLIAFICO, Enrico;
2012

Abstract

In the present work, we analyzed the survival features of six different Epstein–Barr virus (EBV)-stabilized lymphoid cell lines obtained from adult subjects and from subjects of more than 95 years. For the first, we found that lymphoid B cells from centenarians were more resistant to apoptosis induction and displayed a more developed lysosomal compartment, the most critical component of phagic machinery, in comparison with lymphoid B cells from adult subjects. In addition, cells from centenarians were capable of engulfing and digesting other cells, i.e., their siblings (even entire cells), whereas lymphoid cells from “control samples”, i.e., from adults, did not. This behavior was improved by nutrient deprivation but, strikingly, it was unaffected by the autophagy-modulating drug, rapamycin, an autophagy inducer, and 3-methyladenine, an autophagy inhibitor. Transcriptomic analyses indicated that: (1) aspartyl proteases, (2) cell surface molecules such as integrins and cadherins, and (3) some components of cytoskeletal network could contribute to establish this survival phenotype. Also, Kyoto Encyclopedia of Genes and Genomes pathways such as Wnt signaling pathway, an essential contributor to cell migration and actin cytoskeleton remodeling, appeared as prominent. Although we cannot rule out the possibility that EBV-immortalization could play a role, since we observed this phagic behavior in cells from centenarians but not in those from adults, we hypothesize that it may represent an important survival determinant in cells from centenarians.
2012
AGE
34
6
1341
1359
Survival features of EBV-stabilized cells from centenarians: morpho-functional and transcriptomic analyses / Matarrese, P; Tinari, A; Ascione, B; Gambardella, L; Remondini, D; Salvioli, S; Tenedini, Elena; Tagliafico, Enrico; Franceschi, C; Malorni, W.. - In: AGE. - ISSN 1574-4647. - STAMPA. - 34:6(2012), pp. 1341-1359. [10.1007/s11357-011-9307-4]
Matarrese, P; Tinari, A; Ascione, B; Gambardella, L; Remondini, D; Salvioli, S; Tenedini, Elena; Tagliafico, Enrico; Franceschi, C; Malorni, W.
File in questo prodotto:
File Dimensione Formato  
s11357-011-9307-4.pdf

Open access

Tipologia: Versione pubblicata dall'editore
Dimensione 995.29 kB
Formato Adobe PDF
995.29 kB Adobe PDF Visualizza/Apri
Pubblicazioni consigliate

Licenza Creative Commons
I metadati presenti in IRIS UNIMORE sono rilasciati con licenza Creative Commons CC0 1.0 Universal, mentre i file delle pubblicazioni sono rilasciati con licenza Attribuzione 4.0 Internazionale (CC BY 4.0), salvo diversa indicazione.
In caso di violazione di copyright, contattare Supporto Iris

Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11380/707557
Citazioni
  • ???jsp.display-item.citation.pmc??? 3
  • Scopus 7
  • ???jsp.display-item.citation.isi??? 7
social impact